LOCUS       BBa_K1702000            1833 bp    DNA     linear       26-Jan-2026
DEFINITION  Overexpression of E. coli fadD under control of lac promoter
ACCESSION   BBa_K1702000
VERSION     BBa_K1702000.1  
FEATURES             Location/Qualifiers
     CDS             1..1605
                     /label=BBa_K861010
     CDS             1614..1701
                     /label=end of LacI coding region (inactive)
     misc_feature    1614..1813
                     /label=BBa_R0010
     misc_feature    1702..1739
                     /label=CAP binding site
     promoter        1750..1755
                     /label=-35
     promoter        1774..1779
                     /label=-10
     misc_feature    1779..1813
                     /label=LacI binding site
     misc_feature    1786..1786
                     /label=start
     promoter        1614..1813
                     /label=BBa_R0010
     misc_feature    1826..1829
                     /label=conserved
     RBS             1822..1833
                     /label=BBa_B0034
     misc_feature    1614..1833
                     /label=BBa_J04500
ORIGIN
        1 atgtttgagc agtcggtcgc gcgctacgcc gatcaacctg cgtttgtgaa tatgggggag
       61 gtaatgacct tccgcaagct ggaagaacgc agtcgcgcgt ttgccgctta tttgcaacaa
      121 gggttggggc tgaagaaagg cgatcgcgtt gcgttgatga tgcctaattt attgcaatat
      181 ccggtggcgc tgtttggcat tttgcgtgcc gggatgatcg tcgtaaacgt taacccgttg
      241 tataccccgc gtgagcttga gtatcagctt aacgatagcg gcgcatcggc gattgttatc
      301 gtgtctaact ttgctcacac actggaaaaa gtggttgata aaaccgccgt tcagcacgta
      361 attctgaccc gtatgggcga tcagctatct acggcaaaag gcacggtagt caatttcgtt
      421 gttaaataca tcaagcgttt ggtgccgaaa taccatctgc cagatgccat ttcatttcgt
      481 agcgcactgc ataacggcta ccggatgcag tacgtcaaac ccgaactggt gccggaagat
      541 ttagcttttc tgcaatacac cggcggcacc actggtgtgg cgaaaggcgc gatgctgact
      601 caccgcaata tgctggcgaa cctggaacag gttaacgcga cctatggtcc gctgctgcat
      661 ccgggcaaag agctggtggt gacggcgctg ccgctgtatc acatttttgc cctgaccatt
      721 aactgcctgc tgtttatcga actgggtggg cagaacctgc ttatcactaa cccgcgcgat
      781 attccagggt tggtaaaaga gttagcgaaa tatccgttta ccgctatcac gggcgttaac
      841 accttgttca atgcgttgct gaacaataaa gagttccagc agctggattt ctccagtctg
      901 catctttccg caggcggagg gatgccagtg cagcaagtgg tggcagagcg ttgggtgaaa
      961 ctgacaggac agtatctgct ggaaggctat ggccttaccg agtgtgcgcc gctggtcagc
     1021 gttaacccat atgatattga ttatcatagt ggtagcatcg gtttgccggt gccgtcgacg
     1081 gaagccaaac tggtggatga tgatgataat gaagtaccac cgggtcaacc gggtgagctt
     1141 tgtgtcaaag gaccgcaggt gatgctgggt tactggcagc gtccggatgc tacagatgag
     1201 atcatcaaaa atggctggtt acacaccggc gacatcgcgg tgatggatga agaagggttc
     1261 ctgcgcattg tcgatcgtaa aaaagacatg attctggttt ccggttttaa cgtctatccc
     1321 aacgagattg aagatgtcgt catgcagcat cctggcgtac aggaagtcgc ggctgttggc
     1381 gtaccttccg gctccagtgg tgaagcggtg aaaatcttcg tagtgaaaaa agatccatcg
     1441 cttaccgaag agtcactggt gaccttttgc cgccgtcagc tcacgggcta caaagtaccg
     1501 aagctggtgg agtttcgtga tgagttaccg aaatctaacg tcggaaaaat tttgcgacga
     1561 gaattacgtg acgaagcgcg cggcaaagtg gacaataaag cctgatacta gagcaatacg
     1621 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1681 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     1741 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     1801 acaatttcac acatactaga gaaagaggag aaa
//