LOCUS       BBa_K1702002            3178 bp    DNA     linear       15-Jun-2024
DEFINITION  E. coli fadD and fadL under control of lac promoter
ACCESSION   BBa_K1702002
VERSION     BBa_K1702002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1831
                     /label=BBa_K861010
     CDS             1838..3178
                     /label=BBa_K861500
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ttgagcagtc
      241 ggtcgcgcgc tacgccgatc aacctgcgtt tgtgaatatg ggggaggtaa tgaccttccg
      301 caagctggaa gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa
      361 gaaaggcgat cgcgttgcgt tgatgatgcc taatttattg caatatccgg tggcgctgtt
      421 tggcattttg cgtgccggga tgatcgtcgt aaacgttaac ccgttgtata ccccgcgtga
      481 gcttgagtat cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc
      541 tcacacactg gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat
      601 gggcgatcag ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa
      661 gcgtttggtg ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa
      721 cggctaccgg atgcagtacg tcaaacccga actggtgccg gaagatttag cttttctgca
      781 atacaccggc ggcaccactg gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct
      841 ggcgaacctg gaacaggtta acgcgaccta tggtccgctg ctgcatccgg gcaaagagct
      901 ggtggtgacg gcgctgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt
      961 tatcgaactg ggtgggcaga acctgcttat cactaacccg cgcgatattc cagggttggt
     1021 aaaagagtta gcgaaatatc cgtttaccgc tatcacgggc gttaacacct tgttcaatgc
     1081 gttgctgaac aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg
     1141 cggagggatg ccagtgcagc aagtggtggc agagcgttgg gtgaaactga caggacagta
     1201 tctgctggaa ggctatggcc ttaccgagtg tgcgccgctg gtcagcgtta acccatatga
     1261 tattgattat catagtggta gcatcggttt gccggtgccg tcgacggaag ccaaactggt
     1321 ggatgatgat gataatgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc
     1381 gcaggtgatg ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg
     1441 ctggttacac accggcgaca tcgcggtgat ggatgaagaa gggttcctgc gcattgtcga
     1501 tcgtaaaaaa gacatgattc tggtttccgg ttttaacgtc tatcccaacg agattgaaga
     1561 tgtcgtcatg cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc
     1621 cagtggtgaa gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc
     1681 actggtgacc ttttgccgcc gtcagctcac gggctacaaa gtaccgaagc tggtggagtt
     1741 tcgtgatgag ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga
     1801 agcgcgcggc aaagtggaca ataaagcctg atactagatg agccagaaaa ccctgtttac
     1861 aaagtctgct ctcgcagtcg cagtggcact tatctccacc caggcctggt cggcaggctt
     1921 tcagttaaac gaattttctt cctctggcct gggccgggct tattcagggg aaggcgcaat
     1981 tgccgatgat gcaggtaacg ttagccgtaa ccccgcattg attaccatgt ttgaccgccc
     2041 gacattttct gcgggtgcgg tttatattga cccggatgtg aatatcagcg gaacgtctcc
     2101 gtctggtcgt agcctgaaag ccgataacat cgcgcctacg gcatgggttc cgaacatgca
     2161 ctttgttgca ccgattaacg accaatttgg ttggggcgct tctattacct ctaactatgg
     2221 tctggcaaca gagtttaatg atacttatgc aggcggctct gtcgggggta caactgacct
     2281 tgaaaccatg aacctgaact taagcggtgc gtatcgctta aataatgcat ggagctttgg
     2341 tcttggtttc aacgccgtct acgctcgcgc gaaaattgaa cgtttcgcag gcgatctggg
     2401 gcagttggtt gctggtcaga ttatgcaatc tcctgccggg caaactcctc aagggcaagc
     2461 attggcagct accgccaacg gtatcgacag taataccaaa atcgctcatc tgaacggtaa
     2521 ccagtggggc tttggctgga acgccggaat cctgtatgaa ctggataaaa ataaccgcta
     2581 tgcactgacc taccgttctg aagtgaaaat tgacttcaaa ggtaactaca gcagcgatct
     2641 taatcgtgcg tttaataact acggtttgcc aattcctacc gcgacaggtg gcgcaacgca
     2701 atcgggttat ctgacgctga acctgcctga aatgtgggaa gtgtcaggtt ataaccgtgt
     2761 tgatccacag tgggcgattc actatagctt ggcttacacc agctggagtc agttccagca
     2821 gctgaaagcg acctcaacca gtggcgacac gctgttccag aaacatgaag gctttaaaga
     2881 tgcttaccgc atcgcgttgg gtaccactta ttactacgat gataactgga ccttccgtac
     2941 cggtatcgcc tttgatgaca gcccagttcc ggctcagaat cgttctatct ccattccgga
     3001 ccaggaccgt ttctggctga gtgcaggtac gacttacgca tttaataaag atgcttcagt
     3061 cgacgttggt gtttcttata tgcacggtca gagcgtgaaa attaacgaag gcccatacca
     3121 gttcgagtct gaaggtaaag cctggctgtt cggtactaac tttaactacg cgttctga
//