BBa_K1702004
1
BBa_K1702004
trans-zeatin biosynthesis pathway
2015-09-16T11:00:00Z
2015-09-17T03:16:37Z
The genes were synthesized based on sequences based on Kamada-Nobusada, Tomoe and Sakakibara, Hitoshi. "Molecular basis for cytokinin biosynthesis." Phytochemistry 70:444-449. March 2009. and found on KEGG (kegg.jp).
Synthesized tzs gene (from Agrobacterium tumefaciens C58) and LOG (Oryza satifa), both codon optimized for E. coli as a biobrick. The tzs gene encodes for the enzyme which converts 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate into zeatin riboside 5'-phosphate. The LOG gene produces N6-dimethylallyladenine by removing the phosphoribosyl group from N6-(delta2-isopentenyl)-adenosine 5'-monophosphate. Hydroxylation of the N6-dimethylallyladenine produces biologically-active trans-zeatin.
false
false
_2121_
22741
22741
9
false
The piece was synthesized with the two genes functioning as an operon.
false
Olivia Smith
BBa_K1702004_sequence
1
tactagatgctgctgcatctgatctatggtccgacgtgtagtggcaaaacggatatggctattcaaatcgcacaggaaacgggttggccggtggtggcactggatcgtgttcagtgctgtccgcaaatcgctaccggcagtggtcgcccgctggaaagtgaactgcaatccacccgtcgcatttatctggatagccgtccgctgacggaaggcatcctggacgcggaatctgcccatcgtcgcctgatttttgaagttgactggcgcaaatctgaagaaggtctgattctggaaggcggtagcatctctctgctgaactgcatggccaaatcaccgttttggcgttcgggcttccagtggcacgtcaaacgtctgcgcctgggtgatagtgacgcatttctgacgcgtgctaaacaacgcgtggcagaaatgttcgctatccgtgaagatcgcccgtccctgctggaagaactggcggaactgtggaattatccggcggcacgtccgattctggaagatatcgacggctaccgctgtgcgattcgttttgcccgcaaacatgatctggcgatttcacagctgccgaacatcgacgccggtcgtcatgtcgaactgattgaagcaatcgctaatgaatacctggaacacgcgctgtcgcaggaacgcgatttcccgcaatggccggaagatggtgctggtcaaccggtctgcccggtcacgctgacgcgcattcgctgaaggaggaattaaccatggcgatggaagcagcagcagaacgctcggcaggcgcaggtgcagcagcaacggcagccccggaatcaggcggtggtggcgcaggtgaacgtcgctcccgttttcgtcgcatctgcgtgtattgtggtagcgcaaaaggccgcaaagcttcttaccaggatgcggccgtcgaactgggtaaagaactggtggaacgtggcatcgacctggtttatggcggtggctcaattggtctgatgggcctggtttcgcatgcagtccacgatggtggccgccatgtgatcggtgttattccgaaaagcctgatgccgcgtgaagtgaccggtgaaccggtcggcgaagtgcgtgcagtttctggtatgcacgaacgtaaagcggaaatggcccgctttgcagatgctttcattgcgctgccgggtggctatggtaccctggaagaactgctggaagtgatcacgtgggcccaactgggcattcataaaaaaccggtgggtctgctgaacgttgatggcttttacgacccgtttctgagtttcatcgatatggccgtttccgaaggtttcattgcagaagacgctcgtcgcattatcattagcgcaccgacggcacgtgaactggtcctgaaactggaagaatatgttccggaatacgaagtcggcctggtgtgggacgaccaaatgccgcactcgttcgcaccggacctggaaacccgtatcacctcatcctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z