BBa_K1702004 1 BBa_K1702004 trans-zeatin biosynthesis pathway 2015-09-16T11:00:00Z 2015-09-17T03:16:37Z The genes were synthesized based on sequences based on Kamada-Nobusada, Tomoe and Sakakibara, Hitoshi. "Molecular basis for cytokinin biosynthesis." Phytochemistry 70:444-449. March 2009. and found on KEGG (kegg.jp). Synthesized tzs gene (from Agrobacterium tumefaciens C58) and LOG (Oryza satifa), both codon optimized for E. coli as a biobrick. The tzs gene encodes for the enzyme which converts 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate into zeatin riboside 5'-phosphate. The LOG gene produces N6-dimethylallyladenine by removing the phosphoribosyl group from N6-(delta2-isopentenyl)-adenosine 5'-monophosphate. Hydroxylation of the N6-dimethylallyladenine produces biologically-active trans-zeatin. false false _2121_ 22741 22741 9 false The piece was synthesized with the two genes functioning as an operon. false Olivia Smith BBa_K1702004_sequence 1 tactagatgctgctgcatctgatctatggtccgacgtgtagtggcaaaacggatatggctattcaaatcgcacaggaaacgggttggccggtggtggcactggatcgtgttcagtgctgtccgcaaatcgctaccggcagtggtcgcccgctggaaagtgaactgcaatccacccgtcgcatttatctggatagccgtccgctgacggaaggcatcctggacgcggaatctgcccatcgtcgcctgatttttgaagttgactggcgcaaatctgaagaaggtctgattctggaaggcggtagcatctctctgctgaactgcatggccaaatcaccgttttggcgttcgggcttccagtggcacgtcaaacgtctgcgcctgggtgatagtgacgcatttctgacgcgtgctaaacaacgcgtggcagaaatgttcgctatccgtgaagatcgcccgtccctgctggaagaactggcggaactgtggaattatccggcggcacgtccgattctggaagatatcgacggctaccgctgtgcgattcgttttgcccgcaaacatgatctggcgatttcacagctgccgaacatcgacgccggtcgtcatgtcgaactgattgaagcaatcgctaatgaatacctggaacacgcgctgtcgcaggaacgcgatttcccgcaatggccggaagatggtgctggtcaaccggtctgcccggtcacgctgacgcgcattcgctgaaggaggaattaaccatggcgatggaagcagcagcagaacgctcggcaggcgcaggtgcagcagcaacggcagccccggaatcaggcggtggtggcgcaggtgaacgtcgctcccgttttcgtcgcatctgcgtgtattgtggtagcgcaaaaggccgcaaagcttcttaccaggatgcggccgtcgaactgggtaaagaactggtggaacgtggcatcgacctggtttatggcggtggctcaattggtctgatgggcctggtttcgcatgcagtccacgatggtggccgccatgtgatcggtgttattccgaaaagcctgatgccgcgtgaagtgaccggtgaaccggtcggcgaagtgcgtgcagtttctggtatgcacgaacgtaaagcggaaatggcccgctttgcagatgctttcattgcgctgccgggtggctatggtaccctggaagaactgctggaagtgatcacgtgggcccaactgggcattcataaaaaaccggtgggtctgctgaacgttgatggcttttacgacccgtttctgagtttcatcgatatggccgtttccgaaggtttcattgcagaagacgctcgtcgcattatcattagcgcaccgacggcacgtgaactggtcctgaaactggaagaatatgttccggaatacgaagtcggcctggtgtgggacgaccaaatgccgcactcgttcgcaccggacctggaaacccgtatcacctcatcctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z