LOCUS       BBa_K1702005            1709 bp    DNA     linear       01-Jun-2024
DEFINITION  Trans-zeatin biosynthesis under control of the lac promoter
ACCESSION   BBa_K1702005
VERSION     BBa_K1702005.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             229..1709
                     /label=BBa_K1702004
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagta ctagatgctg
      241 ctgcatctga tctatggtcc gacgtgtagt ggcaaaacgg atatggctat tcaaatcgca
      301 caggaaacgg gttggccggt ggtggcactg gatcgtgttc agtgctgtcc gcaaatcgct
      361 accggcagtg gtcgcccgct ggaaagtgaa ctgcaatcca cccgtcgcat ttatctggat
      421 agccgtccgc tgacggaagg catcctggac gcggaatctg cccatcgtcg cctgattttt
      481 gaagttgact ggcgcaaatc tgaagaaggt ctgattctgg aaggcggtag catctctctg
      541 ctgaactgca tggccaaatc accgttttgg cgttcgggct tccagtggca cgtcaaacgt
      601 ctgcgcctgg gtgatagtga cgcatttctg acgcgtgcta aacaacgcgt ggcagaaatg
      661 ttcgctatcc gtgaagatcg cccgtccctg ctggaagaac tggcggaact gtggaattat
      721 ccggcggcac gtccgattct ggaagatatc gacggctacc gctgtgcgat tcgttttgcc
      781 cgcaaacatg atctggcgat ttcacagctg ccgaacatcg acgccggtcg tcatgtcgaa
      841 ctgattgaag caatcgctaa tgaatacctg gaacacgcgc tgtcgcagga acgcgatttc
      901 ccgcaatggc cggaagatgg tgctggtcaa ccggtctgcc cggtcacgct gacgcgcatt
      961 cgctgaagga ggaattaacc atggcgatgg aagcagcagc agaacgctcg gcaggcgcag
     1021 gtgcagcagc aacggcagcc ccggaatcag gcggtggtgg cgcaggtgaa cgtcgctccc
     1081 gttttcgtcg catctgcgtg tattgtggta gcgcaaaagg ccgcaaagct tcttaccagg
     1141 atgcggccgt cgaactgggt aaagaactgg tggaacgtgg catcgacctg gtttatggcg
     1201 gtggctcaat tggtctgatg ggcctggttt cgcatgcagt ccacgatggt ggccgccatg
     1261 tgatcggtgt tattccgaaa agcctgatgc cgcgtgaagt gaccggtgaa ccggtcggcg
     1321 aagtgcgtgc agtttctggt atgcacgaac gtaaagcgga aatggcccgc tttgcagatg
     1381 ctttcattgc gctgccgggt ggctatggta ccctggaaga actgctggaa gtgatcacgt
     1441 gggcccaact gggcattcat aaaaaaccgg tgggtctgct gaacgttgat ggcttttacg
     1501 acccgtttct gagtttcatc gatatggccg tttccgaagg tttcattgca gaagacgctc
     1561 gtcgcattat cattagcgca ccgacggcac gtgaactggt cctgaaactg gaagaatatg
     1621 ttccggaata cgaagtcggc ctggtgtggg acgaccaaat gccgcactcg ttcgcaccgg
     1681 acctggaaac ccgtatcacc tcatcctaa
//