BBa_K1702005
1
BBa_K1702005
Trans-zeatin biosynthesis under control of the lac promoter
2015-09-16T11:00:00Z
2015-09-17T03:35:57Z
This piece was synthesized based on sequences from Kamada-Nobusada, Tomoe and Sakakibara, Hitoshi. "Molecular basis for cytokinin biosynthesis." Phytochemistry 70:444-449. and from KEGG (kegg.jp).
Synthesized tzs gene (from Agrobacterium tumefaciens C58) and LOG (Oryza satifa), both codon optimized for E. coli as a biobrick. The tzs gene encodes for the enzyme which converts 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate into zeatin riboside 5'-phosphate. The LOG gene produces N6-dimethylallyladenine by removing the phosphoribosyl group from N6-(delta2-isopentenyl)-adenosine 5'-monophosphate. Hydroxylation of the N6-dimethylallyladenine produces biologically-active trans-zeatin. Under control of the lac promoter in DH5alpha E. coli cells, expression of the trans-zeatin biosynthesis pathway is induced by lactose or its analog IPTG.
false
false
_2121_
22741
22741
9
false
The piece was synthesized with the two genes back-to-back (i.e. as an operon) and expression can be induced using lactose or IPTG.
false
Olivia Smith, Adriana Collings
component2467153
1
BBa_J04500
component2467154
1
BBa_K1702004
annotation2467153
1
BBa_J04500
range2467153
1
1
220
annotation2467154
1
BBa_K1702004
range2467154
1
229
1709
BBa_J04500
1
BBa_J04500
IPTG inducible promoter with RBS
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
Davidson Synth-Aces
Released HQ 2013
R0010.B0034
false
true
_16_
0
326
16
In stock
false
false
Kristen DeCelle
component1508159
1
BBa_B0034
component1508149
1
BBa_R0010
annotation1508159
1
BBa_B0034
range1508159
1
209
220
annotation1508149
1
BBa_R0010
range1508149
1
1
200
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961225
1
-10
range1961225
1
161
166
BBa_K1702004
1
BBa_K1702004
trans-zeatin biosynthesis pathway
2015-09-16T11:00:00Z
2015-09-17T03:16:37Z
The genes were synthesized based on sequences based on Kamada-Nobusada, Tomoe and Sakakibara, Hitoshi. "Molecular basis for cytokinin biosynthesis." Phytochemistry 70:444-449. March 2009. and found on KEGG (kegg.jp).
Synthesized tzs gene (from Agrobacterium tumefaciens C58) and LOG (Oryza satifa), both codon optimized for E. coli as a biobrick. The tzs gene encodes for the enzyme which converts 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate into zeatin riboside 5'-phosphate. The LOG gene produces N6-dimethylallyladenine by removing the phosphoribosyl group from N6-(delta2-isopentenyl)-adenosine 5'-monophosphate. Hydroxylation of the N6-dimethylallyladenine produces biologically-active trans-zeatin.
false
false
_2121_
22741
22741
9
false
The piece was synthesized with the two genes functioning as an operon.
false
Olivia Smith
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J04500_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_K1702005_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagtactagatgctgctgcatctgatctatggtccgacgtgtagtggcaaaacggatatggctattcaaatcgcacaggaaacgggttggccggtggtggcactggatcgtgttcagtgctgtccgcaaatcgctaccggcagtggtcgcccgctggaaagtgaactgcaatccacccgtcgcatttatctggatagccgtccgctgacggaaggcatcctggacgcggaatctgcccatcgtcgcctgatttttgaagttgactggcgcaaatctgaagaaggtctgattctggaaggcggtagcatctctctgctgaactgcatggccaaatcaccgttttggcgttcgggcttccagtggcacgtcaaacgtctgcgcctgggtgatagtgacgcatttctgacgcgtgctaaacaacgcgtggcagaaatgttcgctatccgtgaagatcgcccgtccctgctggaagaactggcggaactgtggaattatccggcggcacgtccgattctggaagatatcgacggctaccgctgtgcgattcgttttgcccgcaaacatgatctggcgatttcacagctgccgaacatcgacgccggtcgtcatgtcgaactgattgaagcaatcgctaatgaatacctggaacacgcgctgtcgcaggaacgcgatttcccgcaatggccggaagatggtgctggtcaaccggtctgcccggtcacgctgacgcgcattcgctgaaggaggaattaaccatggcgatggaagcagcagcagaacgctcggcaggcgcaggtgcagcagcaacggcagccccggaatcaggcggtggtggcgcaggtgaacgtcgctcccgttttcgtcgcatctgcgtgtattgtggtagcgcaaaaggccgcaaagcttcttaccaggatgcggccgtcgaactgggtaaagaactggtggaacgtggcatcgacctggtttatggcggtggctcaattggtctgatgggcctggtttcgcatgcagtccacgatggtggccgccatgtgatcggtgttattccgaaaagcctgatgccgcgtgaagtgaccggtgaaccggtcggcgaagtgcgtgcagtttctggtatgcacgaacgtaaagcggaaatggcccgctttgcagatgctttcattgcgctgccgggtggctatggtaccctggaagaactgctggaagtgatcacgtgggcccaactgggcattcataaaaaaccggtgggtctgctgaacgttgatggcttttacgacccgtttctgagtttcatcgatatggccgtttccgaaggtttcattgcagaagacgctcgtcgcattatcattagcgcaccgacggcacgtgaactggtcctgaaactggaagaatatgttccggaatacgaagtcggcctggtgtgggacgaccaaatgccgcactcgttcgcaccggacctggaaacccgtatcacctcatcctaa
BBa_K1702004_sequence
1
tactagatgctgctgcatctgatctatggtccgacgtgtagtggcaaaacggatatggctattcaaatcgcacaggaaacgggttggccggtggtggcactggatcgtgttcagtgctgtccgcaaatcgctaccggcagtggtcgcccgctggaaagtgaactgcaatccacccgtcgcatttatctggatagccgtccgctgacggaaggcatcctggacgcggaatctgcccatcgtcgcctgatttttgaagttgactggcgcaaatctgaagaaggtctgattctggaaggcggtagcatctctctgctgaactgcatggccaaatcaccgttttggcgttcgggcttccagtggcacgtcaaacgtctgcgcctgggtgatagtgacgcatttctgacgcgtgctaaacaacgcgtggcagaaatgttcgctatccgtgaagatcgcccgtccctgctggaagaactggcggaactgtggaattatccggcggcacgtccgattctggaagatatcgacggctaccgctgtgcgattcgttttgcccgcaaacatgatctggcgatttcacagctgccgaacatcgacgccggtcgtcatgtcgaactgattgaagcaatcgctaatgaatacctggaacacgcgctgtcgcaggaacgcgatttcccgcaatggccggaagatggtgctggtcaaccggtctgcccggtcacgctgacgcgcattcgctgaaggaggaattaaccatggcgatggaagcagcagcagaacgctcggcaggcgcaggtgcagcagcaacggcagccccggaatcaggcggtggtggcgcaggtgaacgtcgctcccgttttcgtcgcatctgcgtgtattgtggtagcgcaaaaggccgcaaagcttcttaccaggatgcggccgtcgaactgggtaaagaactggtggaacgtggcatcgacctggtttatggcggtggctcaattggtctgatgggcctggtttcgcatgcagtccacgatggtggccgccatgtgatcggtgttattccgaaaagcctgatgccgcgtgaagtgaccggtgaaccggtcggcgaagtgcgtgcagtttctggtatgcacgaacgtaaagcggaaatggcccgctttgcagatgctttcattgcgctgccgggtggctatggtaccctggaagaactgctggaagtgatcacgtgggcccaactgggcattcataaaaaaccggtgggtctgctgaacgttgatggcttttacgacccgtttctgagtttcatcgatatggccgtttccgaaggtttcattgcagaagacgctcgtcgcattatcattagcgcaccgacggcacgtgaactggtcctgaaactggaagaatatgttccggaatacgaagtcggcctggtgtgggacgaccaaatgccgcactcgttcgcaccggacctggaaacccgtatcacctcatcctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z