LOCUS BBa_K1706008 2889 bp DNA linear 25-Nov-2024 DEFINITION HxlR-T7 ACCESSION BBa_K1706008 VERSION BBa_K1706008.1 FEATURES Location/Qualifiers promoter 84..108 /label=A theoretically promoter(very weak) misc_feature 129..158 /label=HxlR protein binding site 1 misc_feature 159..187 /label=HxlR protein binding site 2 promoter 182..208 /label=Formaldehyde promoter RBS 216..225 /label=RBS misc_feature 1..231 /label=BBa_K1706000 promoter 1..231 /label=BBa_K1706000 misc_feature 238..2889 /label=BBa_K1706007 CDS 238..2886 /label=T7 RNA polymerase promoter 238..2889 /label=BBa_K1706007 ORIGIN 1 tagaatcccc cccttagtaa cctttttgta tgttagatac gtatcatcac ttacttactt 61 ttttgcagac tcttttttca ttatactcac tcataaaatc aagcgaaaga aatctgattg 121 tctctcctca cagtatcctc caagtaactt gttgacttca aagtgcctac ttctcaacaa 181 aagaaggaac ctatagaatg aacaataatt cgaaaaaggg agtggatcaa ctactagatg 241 aacacgatta acatcgctaa gaacgacttc tctgacatcg aactggctgc tatcccgttc 301 aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc ccttgagcat 361 gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagcgtca acttaaagct 421 ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact ccctaagatg 481 attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa gcgcccgaca 541 gccttccagt tcctgcaaga aatcaagccg gaagccgtag cgtacatcac cattaagacc 601 actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc aagcgcaatc 661 ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttga agctaagcac 721 ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta caagaaagca 781 tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg cgaggcgtgg 841 tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga gatgctcatt 901 gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg tcaagactct 961 gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc aggtgcgctg 1021 gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg gactggcatt 1081 actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg tactcacagt 1141 aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa agcgattaac 1201 attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc caacgtaatc 1261 accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga agaactcccg 1321 atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa acgtgctgcc 1381 gctgctgtgt accgcaagga cagggctcgc aagtctcgcc gtatcagcct tgagttcatg 1441 cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta caacatggac 1501 tggcgcggtc gtgtttacgc cgtgtcaatg ttcaacccgc aaggtaacga tatgaccaaa 1561 ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta ctggctgaaa 1621 atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg catcaagttc 1681 attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga gaacacttgg 1741 tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta cgctggggta 1801 cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg gtcttgctct 1861 ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc ggttaacttg 1921 cttcctagtg agaccgttca ggacatctac gggattgttg ctaagaaagt caacgagatt 1981 ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac cgatgagaac 2041 actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg tcaatggctg 2101 gctcacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc ttacgggtcc 2161 aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc tattgattcc 2221 ggcaagggtc cgatgttcac tcagccgaat caggctgctg gatacatggc taagctgatt 2281 tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg gcttaagtct 2341 gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat tcttcgcaag 2401 cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga atacaagaag 2461 cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca gcctaccatt 2521 aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat cgctcctaac 2581 tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc acacgagaag 2641 tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc ggctgacgct 2701 gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc ttgtgatgta 2761 ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt ggacaaaatg 2821 ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc ggacttcgcg 2881 ttcgcgtaa //