BBa_K525998
1
pT7
Promoter T7 and RBS
2011-09-12T11:00:00Z
2015-05-08T01:12:35Z
Synthesis
Released HQ 2013
Promoter T7 and RBS
false
false
_690_
0
8543
9
In stock
false
Synthesis
false
Anna Drong
annotation2129432
1
T7 promoter
range2129432
1
1
20
annotation2129433
1
RBS
range2129433
1
24
27
annotation2127788
1
B0034
range2127788
1
21
32
BBa_K1706010
1
BBa_K1706010
T7-PADH-NAD
2015-09-17T11:00:00Z
2015-09-18T05:48:34Z
Artificial synthesis of codon optimized gene
T7 promoter was used to express the formaldehyde dehydrogenase and formate dehydrogenase, and the formaldehyde in the environment was converted to degradation.
false
false
_2126_
20580
20580
9
false
Different hosts, different levels of expression
false
Chaohui Gao
component2474450
1
BBa_K1706002
component2474452
1
BBa_K1706005
component2474449
1
BBa_K525998
annotation2474450
1
BBa_K1706002
range2474450
1
39
1238
annotation2474449
1
BBa_K525998
range2474449
1
1
32
annotation2474452
1
BBa_K1706005
range2474452
1
1245
2339
BBa_K1706005
1
BBa_K1706005
A Synthetic Formate Dehydrogenase Gene
2015-09-17T11:00:00Z
2015-09-18T02:45:36Z
Candida boidinii
FDH, formate dehydrogenase from Candida boidinii can oxidize formate to carbon dioxide in the presence of NAD+.
This is a synthetic formate dehydrogenase gene which codon were optimized using E.coli abundant codon.
Amino acid sequence information reference DQ458777.2.
false
false
_2126_
20580
20580
9
false
The expression of the highest efficiency in E.coli was obtained by using codon preference of E.coli.
false
Chaohui Gao
annotation2470017
1
FDH
range2470017
1
1
1092
annotation2470016
1
BBa_K1706005
range2470016
1
1
1095
BBa_K1706002
1
BBa_K1706002
A Synthetic Formaldehyde Dehydrogenase Gene
2015-09-17T11:00:00Z
2015-09-18T02:18:52Z
Pseudomonas putida W619
PADH, formaldehyde dehydrogenase from Pseudomonas putida is a formaldehyde dehydrogenase which can oxidize free formaldehyde to formate independent of GSH.
This is a synthetic formaldehyde dehydrogenase gene which codon were optimized using E.coli abundant codon.
Amino acid sequence information reference NCBI YP_001751724.1.
false
false
_2126_
20580
20580
9
false
The expression of the highest efficiency in E.coli was obtained by using codon preference of E.coli.
false
Chaohui Gao
annotation2469937
1
BBa_K1706002
range2469937
1
1
1200
annotation2469938
1
Formaldehyde dehydrogenase
range2469938
1
1
1197
BBa_K1706002_sequence
1
atgtcaggaaatcgaggagttgtttatttaggagctggaaaagttgaagttcaaaaaattgattatccaaaaatgcaagatccacgaggaaaaaaaattgaacatggagttattttaaaagttgtttcaactaatatttgtggatcagatcaacatatggttcgaggacgaactactgctcaagttggattagttttaggacatgaaattactggagaaattgttgaaatgggacgagatgttgaacgattaaaaattggagatttagtttcagttccatttaatgttgcttgtggacgatgtcgatcatgtaaagaaatgcatactggagtttgtttaactgttaatccagctcgagctggaggagcttatggatatgttgatatgggagattggactggaggacaagctgaatatgttttagttccatatgctgattttaatttattaaaattaccagatcgagataaagctatggaaaaaattcgagatttaacttgtttatcagatattttaccaactggatatcatggagctgttactgctggagttggaccaggatcaactgtttatgttgctggagctggaccagttggattagctgctgctgcttcagctcgattattaggagctgcttgtgttattgttggagatttaaatccagctcgattagctcatgctaaatcacaaggatttgaagttgttgatttatcaaaagatactccattacatgaacaaattattgatattttaggagaaccagaagttgattgtgctgttgatgctgttggatttgaagctcgaggacatggacatgaaggagctaaacatgaagctccagctactgttttaaattcattaatgcaagttactcgagttgctggaaatattggagttccaggattatatgttactgaagatccaggagctgttgatgctgctgctaaaattggagctttatcaattcgatttggattaggatgggctaaatcacattcatttcatactggacaaactccaactatgaaatataatcgacaattaatgcaagctattatgtgggatcgaattaatattgctgaagttgttggagttcaagttatttcattagatcaagctccagaaggatatggagaatttgatgctggagttccaaaaaaatttgttattgatccacataaaactttttcagctgcttaa
BBa_K525998_sequence
1
taatacgactcactatagggaaagaggagaaa
BBa_K1706010_sequence
1
taatacgactcactatagggaaagaggagaaatactagatgtcaggaaatcgaggagttgtttatttaggagctggaaaagttgaagttcaaaaaattgattatccaaaaatgcaagatccacgaggaaaaaaaattgaacatggagttattttaaaagttgtttcaactaatatttgtggatcagatcaacatatggttcgaggacgaactactgctcaagttggattagttttaggacatgaaattactggagaaattgttgaaatgggacgagatgttgaacgattaaaaattggagatttagtttcagttccatttaatgttgcttgtggacgatgtcgatcatgtaaagaaatgcatactggagtttgtttaactgttaatccagctcgagctggaggagcttatggatatgttgatatgggagattggactggaggacaagctgaatatgttttagttccatatgctgattttaatttattaaaattaccagatcgagataaagctatggaaaaaattcgagatttaacttgtttatcagatattttaccaactggatatcatggagctgttactgctggagttggaccaggatcaactgtttatgttgctggagctggaccagttggattagctgctgctgcttcagctcgattattaggagctgcttgtgttattgttggagatttaaatccagctcgattagctcatgctaaatcacaaggatttgaagttgttgatttatcaaaagatactccattacatgaacaaattattgatattttaggagaaccagaagttgattgtgctgttgatgctgttggatttgaagctcgaggacatggacatgaaggagctaaacatgaagctccagctactgttttaaattcattaatgcaagttactcgagttgctggaaatattggagttccaggattatatgttactgaagatccaggagctgttgatgctgctgctaaaattggagctttatcaattcgatttggattaggatgggctaaatcacattcatttcatactggacaaactccaactatgaaatataatcgacaattaatgcaagctattatgtgggatcgaattaatattgctgaagttgttggagttcaagttatttcattagatcaagctccagaaggatatggagaatttgatgctggagttccaaaaaaatttgttattgatccacataaaactttttcagctgcttaatactagatgaaaattgttttagttttatatggagctggaaaacatgctgctgatgaagaaaaattatatggatgtactgaaaataaattaggaattgctaattggttaaaagatcaaggacatgaattaattactacttcagataaagaaggaggaaattcagttttagatcaacatattccagatgctgatattattattactactccatttcatccagcttatattactaaagaacgaattgataaagctaaaaaattaaaattagttgttgttgctggagttggatcagatcatattgatttagattatattaatcaaactggacgaaaaatttcagttttagaagttactggatcaaatgttgtttcagttgctgaacatgttgttatgactatgttagttttagttcgaaattttgttccagctcatgaacaaaatattaatcatgattgggaagttgctgctattgctaaagatgcttatgatattgaaggaaaaactattgctactattggagctggacgaattggatatcgagttttagaacgattagttccatttaatccaaaagaattattatattatgattatcaagctttaccaaaagatgctgaagaaaaagttggagctcgacgagttgaaaatattgaagaattagttgctcaagctgatattgttactgttaatgctccattacatgctggaactaaaggattaattaataaagaattattatcaaaatttaaaaaaggagcttggttagttaatactgctcgaggagctatttgtgttgctgaagatgttgctgctgctttagaatcaggacaattacgaggatatggaggagatgtttggtttccacaaccagctccaaaagatcatccatggcgagatatgcgaaataaatatggagctggaaatgctactactccacattattcaggaactactttagatgctcaaactcgatatgctcaaggaactaaaaatattttagaatcattttttactggaaaatttgattatcgaccacaagatattattttattaaatggagaatatgttactaaagcttatggaaaacatgataaaaaataa
BBa_K1706005_sequence
1
atgaaaattgttttagttttatatggagctggaaaacatgctgctgatgaagaaaaattatatggatgtactgaaaataaattaggaattgctaattggttaaaagatcaaggacatgaattaattactacttcagataaagaaggaggaaattcagttttagatcaacatattccagatgctgatattattattactactccatttcatccagcttatattactaaagaacgaattgataaagctaaaaaattaaaattagttgttgttgctggagttggatcagatcatattgatttagattatattaatcaaactggacgaaaaatttcagttttagaagttactggatcaaatgttgtttcagttgctgaacatgttgttatgactatgttagttttagttcgaaattttgttccagctcatgaacaaaatattaatcatgattgggaagttgctgctattgctaaagatgcttatgatattgaaggaaaaactattgctactattggagctggacgaattggatatcgagttttagaacgattagttccatttaatccaaaagaattattatattatgattatcaagctttaccaaaagatgctgaagaaaaagttggagctcgacgagttgaaaatattgaagaattagttgctcaagctgatattgttactgttaatgctccattacatgctggaactaaaggattaattaataaagaattattatcaaaatttaaaaaaggagcttggttagttaatactgctcgaggagctatttgtgttgctgaagatgttgctgctgctttagaatcaggacaattacgaggatatggaggagatgtttggtttccacaaccagctccaaaagatcatccatggcgagatatgcgaaataaatatggagctggaaatgctactactccacattattcaggaactactttagatgctcaaactcgatatgctcaaggaactaaaaatattttagaatcattttttactggaaaatttgattatcgaccacaagatattattttattaaatggagaatatgttactaaagcttatggaaaacatgataaaaaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z