##gff-version 3
##sequence-region BBa_K1707005 1 1870
BBa_K1707005	.	engineered_region	44	746	.	+	0	ID=BBa_S03518;Name=BBa_S03518
BBa_K1707005	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03518
BBa_K1707005	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1707005	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S03518
BBa_K1707005	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1707005	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1707005	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1707005	.	engineered_region	892	1870	.	+	0	ID=BBa_I13602;Name=BBa_I13602
BBa_K1707005	.	terminator	1830	1870	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13602
BBa_K1707005	.	polyA_site	1857	1870	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1707005	.	stem_loop	1837	1856	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1707005	.	engineered_region	1830	1870	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1707005	.	stop_codon	1863	1863	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1707005	.	terminator	1742	1821	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13602
BBa_K1707005	.	stem_loop	1753	1796	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1707005	.	engineered_region	1742	1821	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1707005	.	engineered_region	972	1733	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_I13602
BBa_K1707005	.	tag	1690	1727	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_K1707005	.	CDS	972	1733	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_K1707005	.	stop_codon	1728	1733	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_K1707005	.	engineered_region	972	1733	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_K1707005	.	point_mutation	1040	1040	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_K1707005	.	ribosome_entry_site	954	965	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13602
BBa_K1707005	.	conserved_region	958	961	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1707005	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13602
BBa_K1707005	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1707005	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1707005	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1707005	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1707005	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1707005	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1707005	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1707005	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1707005	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1707005	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1707005	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1707005	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1707005	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1707005	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1707005	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101
>BBa_K1707005
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagct
ggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctg
aagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgct
tcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcac
catcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatc
gagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacg
tctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgt
gcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagc
acccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccggga
tcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata