LOCUS       BBa_K1707021            1022 bp    DNA     linear       29-Nov-2024
DEFINITION  constitutive promoter, RNA thermometer, GFP-ssRA
ACCESSION   BBa_K1707021
VERSION     BBa_K1707021.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             120..125
                     /label=SD
     misc_feature    44..126
                     /label=BBa_K115017
     misc_feature    133..135
                     /label=start
     CDS             136..846
                     /label=GFP (mut3b)
     misc_feature    847..879
                     /label=AAV-ssrA tag
     misc_feature    880..882
                     /label=stop
     misc_feature    883..885
                     /label=stop
     CDS             133..885
                     /label=BBa_K1399005
     stem_loop       905..948
                     /label=stem_loop
     misc_feature    894..973
                     /label=BBa_B0010
     terminator      894..973
                     /label=BBa_B0010
     stem_loop       989..1008
                     /label=T7 TE
     misc_feature    1015..1015
                     /label=stop
     polyA_site      1009..1022
                     /label=polya
     misc_feature    982..1022
                     /label=BBa_B0012
     terminator      982..1022
                     /label=BBa_B0012
     terminator      894..1022
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagccgggcg cccttcgggg
       61 gcccggcgga gacgggcgcc ggaggtgtcc gacgcctgct cgtccagtct ttgctcagtg
      121 gaggattact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      181 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      241 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      301 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
      361 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      421 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      481 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      541 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      601 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
      661 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      721 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
      781 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
      841 tacaaagctg caaacgacga aaactacgct gctgctgttt aataatacta gagccaggca
      901 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      961 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1021 ta
//