LOCUS       BBa_K1707022             968 bp    DNA     linear       22-Dec-2024
DEFINITION  cI promoter withGFP-ssrA
ACCESSION   BBa_K1707022
VERSION     BBa_K1707022.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..72
                     /label=RBS-1\Strong
     RBS             65..69
                     /label=RBS
     misc_feature    58..72
                     /label=BBa_B0030
     RBS             58..72
                     /label=BBa_B0030
     misc_feature    79..81
                     /label=start
     CDS             82..792
                     /label=GFP (mut3b)
     misc_feature    793..825
                     /label=AAV-ssrA tag
     misc_feature    826..828
                     /label=stop
     misc_feature    829..831
                     /label=stop
     CDS             79..831
                     /label=BBa_K1399005
     stem_loop       851..894
                     /label=stem_loop
     misc_feature    840..919
                     /label=BBa_B0010
     terminator      840..919
                     /label=BBa_B0010
     stem_loop       935..954
                     /label=T7 TE
     misc_feature    961..961
                     /label=stop
     polyA_site      955..968
                     /label=polya
     misc_feature    928..968
                     /label=BBa_B0012
     terminator      928..968
                     /label=BBa_B0012
     terminator      840..968
                     /label=BBa_B0015
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt
       61 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
      121 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      181 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      241 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      301 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      361 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      421 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      481 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      541 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      601 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      661 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      721 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      781 gaactataca aagctgcaaa cgacgaaaac tacgctgctg ctgtttaata atactagagc
      841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      961 cgtttata
//