LOCUS       BBa_K1707029            1897 bp    DNA     linear       22-Dec-2024
DEFINITION  thermosensitive cI repressor controlling the expression of CFP-ssRA
ACCESSION   BBa_K1707029
VERSION     BBa_K1707029.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..778
                     /label=cIts
     CDS             65..778
                     /label=BBa_K098997
     stem_loop       798..841
                     /label=stem_loop
     misc_feature    787..866
                     /label=BBa_B0010
     terminator      787..866
                     /label=BBa_B0010
     stem_loop       882..901
                     /label=T7 TE
     misc_feature    908..908
                     /label=stop
     polyA_site      902..915
                     /label=polya
     misc_feature    875..915
                     /label=BBa_B0012
     terminator      875..915
                     /label=BBa_B0012
     terminator      787..915
                     /label=BBa_B0015
     misc_feature    1..915
                     /label=BBa_K1707023
     promoter        961..966
                     /label=-10
     promoter        938..943
                     /label=-35
     misc_feature    948..964
                     /label=OR1
     misc_feature    924..940
                     /label=OR2
     misc_feature    924..972
                     /label=BBa_R0051
     promoter        924..972
                     /label=BBa_R0051
     misc_feature    985..988
                     /label=conserved
     RBS             981..992
                     /label=BBa_B0034
     CDS             999..1760
                     /label=CFP (LVA)
     misc_feature    1717..1754
                     /label=SsrA
     misc_feature    1755..1760
                     /label=2
     misc_feature    1067..1067
                     /label=A
     misc_feature    999..1760
                     /label=BBa_E0022
     misc_feature    999..1760
                     /label=BBa_E0022
     stem_loop       1780..1823
                     /label=stem_loop
     misc_feature    1769..1848
                     /label=BBa_B0010
     terminator      1769..1848
                     /label=BBa_B0010
     stem_loop       1864..1883
                     /label=T7 TE
     misc_feature    1890..1890
                     /label=stop
     polyA_site      1884..1897
                     /label=polya
     misc_feature    1857..1897
                     /label=BBa_B0012
     terminator      1857..1897
                     /label=BBa_B0012
     misc_feature    981..1897
                     /label=BBa_E0422
     misc_feature    924..1897
                     /label=BBa_K1707028
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagattaaag aggagaaata
       61 ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct
      121 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga
      181 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa
      241 tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc
      301 ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag
      361 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct
      421 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga
      481 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc
      541 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga
      601 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag
      661 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag
      721 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggctgata
      781 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      841 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      901 ctttctgcgt ttatatacta gagtaacacc gtgcgtgttg actattttac ctctggcggt
      961 gataatggtt gctactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc
     1021 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac ggccacaagt
     1081 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1141 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
     1201 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1261 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1321 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1381 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca
     1441 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     1501 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1561 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1621 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1681 ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac gacgaaaact
     1741 acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1801 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1861 actggctcac cttcgggtgg gcctttctgc gtttata
//