LOCUS       BBa_K1707032            1694 bp    DNA     linear       22-Nov-2024
DEFINITION  tetR-ssRA controlling the expression of CFP
ACCESSION   BBa_K1707032
VERSION     BBa_K1707032.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..746
                     /label=BBa_C0040
     misc_feature    44..746
                     /label=BBa_S03518
     misc_feature    1..746
                     /label=BBa_K1707008
     misc_feature    755..773
                     /label=TetR 1
     misc_feature    755..808
                     /label=BBa_R0040
     promoter        774..779
                     /label=-35
     misc_feature    780..798
                     /label=TetR 2
     promoter        797..802
                     /label=-10
     promoter        755..808
                     /label=BBa_R0040
     misc_feature    821..824
                     /label=conserved
     RBS             817..828
                     /label=BBa_B0034
     CDS             835..1557
                     /label=BBa_E0020
     stem_loop       1577..1620
                     /label=stem_loop
     misc_feature    1566..1645
                     /label=BBa_B0010
     terminator      1566..1645
                     /label=BBa_B0010
     stem_loop       1661..1680
                     /label=T7 TE
     misc_feature    1687..1687
                     /label=stop
     polyA_site      1681..1694
                     /label=polya
     misc_feature    1654..1694
                     /label=BBa_B0012
     terminator      1654..1694
                     /label=BBa_B0012
     misc_feature    755..1694
                     /label=BBa_I13600
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagtcccta tcagtgatag agattgacat
      781 ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata ctagatggtg
      841 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      901 gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      961 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1021 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1081 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1141 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1201 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1261 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
     1321 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1381 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1441 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1501 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataatac
     1561 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1621 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1681 tttctgcgtt tata
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