##gff-version 3
##sequence-region BBa_K1707032 1 1694
BBa_K1707032	.	engineered_region	1	746	.	+	0	ID=BBa_K1707008;Name=BBa_K1707008
BBa_K1707032	.	engineered_region	44	746	.	+	0	ID=BBa_S03518;Name=BBa_S03518;Parent=BBa_K1707008
BBa_K1707032	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03518
BBa_K1707032	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1707032	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S03518
BBa_K1707032	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1707032	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1707032	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1707032	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1707008
BBa_K1707032	.	engineered_region	755	1694	.	+	0	ID=BBa_I13600;Name=BBa_I13600
BBa_K1707032	.	promoter	755	808	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13600
BBa_K1707032	.	promoter	774	779	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1707032	.	engineered_region	755	808	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1707032	.	non_covalent_binding_site	755	773	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1707032	.	promoter	797	802	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1707032	.	non_covalent_binding_site	780	798	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1707032	.	CDS	835	1557	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_I13600
BBa_K1707032	.	terminator	1566	1645	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13600
BBa_K1707032	.	engineered_region	1566	1645	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1707032	.	stem_loop	1577	1620	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1707032	.	terminator	1654	1694	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13600
BBa_K1707032	.	polyA_site	1681	1694	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1707032	.	engineered_region	1654	1694	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1707032	.	stop_codon	1687	1687	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1707032	.	stem_loop	1661	1680	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1707032	.	ribosome_entry_site	817	828	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13600
BBa_K1707032	.	conserved_region	821	824	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1707032
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagt
gatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggt
ggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcc
acctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccc
tgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccga
aggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggc
gacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagt
acaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgcca
caacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctg
cccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctgg
agttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata