LOCUS       BBa_K1707037            1958 bp    DNA     linear       22-Dec-2024
DEFINITION  cI repressor controlling the expression of CFP-ssRA
ACCESSION   BBa_K1707037
VERSION     BBa_K1707037.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             68..775
                     /label=cI lambda
     misc_feature    776..808
                     /label=LVA
     misc_feature    815..839
                     /label=Help:Barcodes
     CDS             65..839
                     /label=BBa_C0051
     stem_loop       859..902
                     /label=stem_loop
     misc_feature    848..927
                     /label=BBa_B0010
     terminator      848..927
                     /label=BBa_B0010
     stem_loop       943..962
                     /label=T7 TE
     misc_feature    969..969
                     /label=stop
     polyA_site      963..976
                     /label=polya
     misc_feature    936..976
                     /label=BBa_B0012
     terminator      936..976
                     /label=BBa_B0012
     terminator      848..976
                     /label=BBa_B0015
     misc_feature    1..976
                     /label=BBa_K1707034
     promoter        1022..1027
                     /label=-10
     promoter        999..1004
                     /label=-35
     misc_feature    1009..1025
                     /label=OR1
     misc_feature    985..1001
                     /label=OR2
     misc_feature    985..1033
                     /label=BBa_R0051
     promoter        985..1033
                     /label=BBa_R0051
     misc_feature    1046..1049
                     /label=conserved
     RBS             1042..1053
                     /label=BBa_B0034
     CDS             1060..1821
                     /label=CFP (LVA)
     misc_feature    1778..1815
                     /label=SsrA
     misc_feature    1816..1821
                     /label=2
     misc_feature    1128..1128
                     /label=A
     misc_feature    1060..1821
                     /label=BBa_E0022
     misc_feature    1060..1821
                     /label=BBa_E0022
     stem_loop       1841..1884
                     /label=stem_loop
     misc_feature    1830..1909
                     /label=BBa_B0010
     terminator      1830..1909
                     /label=BBa_B0010
     stem_loop       1925..1944
                     /label=T7 TE
     misc_feature    1951..1951
                     /label=stop
     polyA_site      1945..1958
                     /label=polya
     misc_feature    1918..1958
                     /label=BBa_B0012
     terminator      1918..1958
                     /label=BBa_B0012
     misc_feature    1042..1958
                     /label=BBa_E0422
     misc_feature    985..1958
                     /label=BBa_K1707028
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagattaaag aggagaaata
       61 ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct
      121 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga
      181 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa
      241 tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc
      301 ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag
      361 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct
      421 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga
      481 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc
      541 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga
      601 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag
      661 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag
      721 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc
      781 aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct
      841 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      901 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      961 cctttctgcg tttatatact agagtaacac cgtgcgtgtt gactatttta cctctggcgg
     1021 tgataatggt tgctactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag
     1081 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtgaa cggccacaag
     1141 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1201 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
     1261 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1321 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1381 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1441 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc
     1501 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     1561 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1621 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1681 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1741 gccgggatca ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac
     1801 tacgctttag tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1861 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1921 cactggctca ccttcgggtg ggcctttctg cgtttata
//