BBa_K1709004
1
BBa_K1709004
LuxR-E
2015-09-17T11:00:00Z
2015-09-18T11:21:02Z
Sequence is originating from V. fischeri. Here, the sequence from BBa_I0462 was used and optimized.
Encodes a transcription activator for the right handed Lux promotor.
Two molecules of acyl-homoserine lactones (AHL) form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri.
false
false
_2129_
25389
25389
9
false
Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. For our own use, 9 bp were exchanged to create unique restriction sites and to optimize DNA folding.
false
Astrid Deryckere
annotation2470868
1
LuxR
range2470868
1
1
750
annotation2470849
1
Ochre Stop Codon
range2470849
1
799
801
annotation2470847
1
ATG Start Codon
range2470847
1
1
3
annotation2470866
1
E-tag
range2470866
1
753
801
BBa_K1709004_sequence
1
atgaaaaacataaatgccgacgacacatacagaataatcaacaaaatcaaagcttgtagaagcaataatgatatcaatcaatgcttgtctgatatgactaaaatggtccattgcgaatattatttactcgcgatcatttatccacactctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattcaggtgcgccggtgccgtatccggatccgctggaaccgcgtgccgcataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z