LOCUS       BBa_K1709006            1559 bp    DNA     linear       24-Apr-2024
DEFINITION  RBS-LuxI-His-RBS-LuxR-E-Term-Plux
ACCESSION   BBa_K1709006
VERSION     BBa_K1709006.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=BBa_J61104
     misc_feature    19..21
                     /label=ATG Start Codon
     CDS             19..597
                     /label=LuxI Coding Region
     misc_feature    601..624
                     /label=Polyhistidine-tag
     misc_feature    625..627
                     /label=Ochre Stop Codon
     CDS             19..627
                     /label=BBa_K1709000
     misc_feature    1..627
                     /label=BBa_K1709001
     RBS             636..647
                     /label=BBa_J61100
     misc_feature    654..656
                     /label=ATG Start Codon
     misc_feature    1452..1454
                     /label=Ochre Stop Codon
     misc_feature    1406..1454
                     /label=E-tag
     CDS             654..1403
                     /label=LuxR
     CDS             654..1454
                     /label=BBa_K1709004
     misc_feature    1463..1496
                     /label=B1002
     stem_loop       1472..1487
                     /label=stem loop
     polyA_site      1487..1493
                     /label=Poly A tail
     polyA_site      1466..1471
                     /label=Poly A tail
     terminator      1463..1496
                     /label=BBa_B1002
     misc_feature    1505..1524
                     /label=LuxR/HSL
     promoter        1524..1529
                     /label=-35
     promoter        1546..1551
                     /label=-10
     misc_feature    1557..1557
                     /label=start
     misc_feature    1505..1559
                     /label=BBa_R0062
     promoter        1505..1559
                     /label=BBa_R0062
ORIGIN
        1 aaagaaggga catactagat gactataatg ataaaaaaat cggatttttt ggcaattcca
       61 tcggaagagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag
      121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa
      181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca
      241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc
      301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata
      361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt
      421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag
      481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact
      541 aaatcggttg tattatccat gcccatcaat gaacagttta aaaaagcagt cttaaattca
      601 caccatcacc atcaccatgc cgcataatac tagagaaaga ggggacatac tagatgaaaa
      661 acataaatgc cgacgacaca tacagaataa tcaacaaaat caaagcttgt agaagcaata
      721 atgatatcaa tcaatgcttg tctgatatga ctaaaatggt ccattgcgaa tattatttac
      781 tcgcgatcat ttatccacac tctatggtta aatctgatat ttcaatccta gataattacc
      841 ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag
      901 attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa
      961 ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta
     1021 gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa
     1081 aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc
     1141 cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt
     1201 taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata
     1261 tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa
     1321 tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa
     1381 ttgattgccc atactttaaa aattcaggtg cgccggtgcc gtatccggat ccgctggaac
     1441 cgcgtgccgc ataatactag agcgcaaaaa accccgcttc ggcggggttt tttcgctact
     1501 agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgaataaa
//