BBa_K1709006
type: ComponentDefinition
persistent identity: BBa K1709006
display id: BBa_K1709006
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-LuxI-His-RBS-LuxR-E-Term-Plux
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:29:08Z
mutable provenance: The Lux operon originates from V. Fischeri.
owned by: james
owned by: myers
mutable description: Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right Lux promotor only gives weak constitutive expression of downstream genes. Any gene you would like to be controlled by AHL and thus cell density can be cloned after this complex.
discontinued: false
dominant: false
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: For the ease of cloning, the sequence was devided in multiple gBlocks. Therefor, there is a mistake in the composite part sequence since the scar is only partly present.
star: false
creator: Astrid Deryckere
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_R0062
component: BBa_B1002
component: BBa_K1709004
component: BBa_J61100
component: BBa_K1709001
sequence annotation: BBa_K1709001
sequence annotation: BBa_J61100
sequence annotation: BBa_K1709004
sequence annotation: BBa_B1002
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1709006_sequence/1

BBa_R0062
type: Component
persistent identity: component2473343
display id: component2473343
version: 1
top level: BBa_K1709006
definition: lux pR
access: public

BBa_B1002
type: Component
persistent identity: component2473341
display id: component2473341
version: 1
top level: BBa_K1709006
definition: BBa_B1002
access: public

BBa_K1709004
type: Component
persistent identity: component2473336
display id: component2473336
version: 1
top level: BBa_K1709006
definition: BBa_K1709004
access: public

BBa_J61100
type: Component
persistent identity: component2473331
display id: component2473331
version: 1
top level: BBa_K1709006
definition: BBa_J61100
access: public

BBa_K1709001
type: Component
persistent identity: component2473330
display id: component2473330
version: 1
top level: BBa_K1709006
definition: BBa_K1709001
access: public

BBa_K1709001
type: SequenceAnnotation
persistent identity: annotation2473330
display id: annotation2473330
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473330/range2473330/1
component: BBa_K1709001

https://synbiohub.org/public/igem/BBa_K1709006/annotation2473330/range2473330/1
type: Range
persistent identity: range2473330
display id: range2473330
version: 1
top level: BBa_K1709006
direction: direction/reverse
start: 1
end: 627
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2473331
display id: annotation2473331
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473331/range2473331/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K1709006/annotation2473331/range2473331/1
type: Range
persistent identity: range2473331
display id: range2473331
version: 1
top level: BBa_K1709006
direction: direction/reverse
start: 636
end: 647
orientation: inline

BBa_K1709004
type: SequenceAnnotation
persistent identity: annotation2473336
display id: annotation2473336
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473336/range2473336/1
component: BBa_K1709004

https://synbiohub.org/public/igem/BBa_K1709006/annotation2473336/range2473336/1
type: Range
persistent identity: range2473336
display id: range2473336
version: 1
top level: BBa_K1709006
direction: direction/reverse
start: 654
end: 1454
orientation: inline

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2473341
display id: annotation2473341
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473341/range2473341/1
component: BBa_B1002

https://synbiohub.org/public/igem/BBa_K1709006/annotation2473341/range2473341/1
type: Range
persistent identity: range2473341
display id: range2473341
version: 1
top level: BBa_K1709006
direction: direction/reverse
start: 1463
end: 1496
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2473343
display id: annotation2473343
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473343/range2473343/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1709006/annotation2473343/range2473343/1
type: Range
persistent identity: range2473343
display id: range2473343
version: 1
top level: BBa_K1709006
direction: direction/forward
start: 1505
end: 1559
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

BBa_B1002
type: ComponentDefinition
persistent identity: BBa B1002
display id: BBa_B1002
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, small, %T~=85%)
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
mutable description: Artifical terminator, estimated %T~=85
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1002
sequence annotation: Poly A tail
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1002_sequence/1

B1002
type: SequenceAnnotation
persistent identity: annotation1898412
display id: annotation1898412
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
type: Range
persistent identity: range1898412
display id: range1898412
version: 1
top level: BBa_B1002
direction: direction/forward
start: 1
end: 34
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898415
display id: annotation1898415
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
type: Range
persistent identity: range1898415
display id: range1898415
version: 1
top level: BBa_B1002
direction: direction/reverse
start: 4
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898413
display id: annotation1898413
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
type: Range
persistent identity: range1898413
display id: range1898413
version: 1
top level: BBa_B1002
direction: direction/forward
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898414
display id: annotation1898414
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
type: Range
persistent identity: range1898414
display id: range1898414
version: 1
top level: BBa_B1002
direction: direction/forward
start: 25
end: 31
orientation: inline

BBa_K1709001
type: ComponentDefinition
persistent identity: BBa K1709001
display id: BBa_K1709001
version: 1
was generated by: iGEM to SBOL conversion
description: LuxI-His
created: 2015-09-07T11:00:00Z
modified: 2015-09-26T09:17:10Z
mutable provenance: Sequence is originating from V. fischeri. Here, the sequence from BBa_C0061 was used and optimized.
owned by: james
owned by: myers
mutable description: Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right lux promotor only gives weak constitutive expression of downstream genes. The LuxI gene is fused to a His-tag facilitating gene expression measurements.
discontinued: false
dominant: false
experience: experience/None
group u list: _2129_
m user id: 25069
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. Also, a Ribosome Binding Site was added. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.
star: false
creator: Astrid Deryckere
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J61104
component: BBa_K1709000
sequence annotation: BBa_J61104
sequence annotation: BBa_K1709000
sequence: https://synbiohub.org/public/igem/BBa_K1709001_sequence/1

BBa_J61104
type: Component
persistent identity: component2473563
display id: component2473563
version: 1
top level: BBa_K1709001
definition: BBa_J61104
access: public

BBa_K1709000
type: Component
persistent identity: component2473568
display id: component2473568
version: 1
top level: BBa_K1709001
definition: LuxI-His
access: public

BBa_J61104
type: SequenceAnnotation
persistent identity: annotation2473563
display id: annotation2473563
version: 1
top level: BBa_K1709001
location: https://synbiohub.org/public/igem/BBa_K1709001/annotation2473563/range2473563/1
component: BBa_J61104

https://synbiohub.org/public/igem/BBa_K1709001/annotation2473563/range2473563/1
type: Range
persistent identity: range2473563
display id: range2473563
version: 1
top level: BBa_K1709001
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1709000
type: SequenceAnnotation
persistent identity: annotation2473568
display id: annotation2473568
version: 1
top level: BBa_K1709001
location: https://synbiohub.org/public/igem/BBa_K1709001/annotation2473568/range2473568/1
component: BBa_K1709000

https://synbiohub.org/public/igem/BBa_K1709001/annotation2473568/range2473568/1
type: Range
persistent identity: range2473568
display id: range2473568
version: 1
top level: BBa_K1709001
direction: direction/reverse
start: 19
end: 627
orientation: inline

BBa_K1709004
type: ComponentDefinition
persistent identity: BBa K1709004
display id: BBa_K1709004
version: 1
was generated by: iGEM to SBOL conversion
description: LuxR-E
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:21:02Z
mutable provenance: Sequence is originating from V. fischeri. Here, the sequence from BBa_I0462 was used and optimized.
owned by: james
owned by: myers
mutable description: Encodes a transcription activator for the right handed Lux promotor. Two molecules of acyl-homoserine lactones (AHL) form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri.
discontinued: false
dominant: false
experience: experience/None
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. For our own use, 9 bp were exchanged to create unique restriction sites and to optimize DNA folding.
star: false
creator: Astrid Deryckere
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ATG Start Codon
sequence annotation: LuxR
sequence annotation: E-tag
sequence annotation: Ochre Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K1709004_sequence/1

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2470847
display id: annotation2470847
version: 1
top level: BBa_K1709004
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470847/range2470847/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470847/range2470847/1
type: Range
persistent identity: range2470847
display id: range2470847
version: 1
top level: BBa_K1709004
direction: direction/forward
start: 1
end: 3
orientation: inline

LuxR
type: SequenceAnnotation
persistent identity: annotation2470868
display id: annotation2470868
version: 1
top level: BBa_K1709004
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470868/range2470868/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470868/range2470868/1
type: Range
persistent identity: range2470868
display id: range2470868
version: 1
top level: BBa_K1709004
direction: direction/forward
start: 1
end: 750
orientation: inline

E-tag
type: SequenceAnnotation
persistent identity: annotation2470866
display id: annotation2470866
version: 1
top level: BBa_K1709004
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470866/range2470866/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470866/range2470866/1
type: Range
persistent identity: range2470866
display id: range2470866
version: 1
top level: BBa_K1709004
direction: direction/forward
start: 753
end: 801
orientation: inline

Ochre Stop Codon
type: SequenceAnnotation
persistent identity: annotation2470849
display id: annotation2470849
version: 1
top level: BBa_K1709004
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470849/range2470849/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470849/range2470849/1
type: Range
persistent identity: range2470849
display id: range2470849
version: 1
top level: BBa_K1709004
direction: direction/forward
start: 799
end: 801
orientation: inline

LuxI-His
type: ComponentDefinition
persistent identity: BBa K1709000
display id: BBa_K1709000
version: 1
was generated by: iGEM to SBOL conversion
description: LuxI-His
created: 2015-09-07T11:00:00Z
modified: 2015-09-18T04:12:52Z
mutable provenance: Sequence is originating from V. fischeri. Here, the sequence from BBa_C0061 was used and optimized.
owned by: james
owned by: myers
mutable description: Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right lux promotor only gives weak constitutive expression of downstream genes. The LuxI gene is fused to a His-tag facilitating gene expression measurements.
discontinued: false
dominant: false
experience: experience/None
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.
star: false
creator: Astrid Deryckere
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: LuxI Coding Region
sequence annotation: ATG Start Codon
sequence annotation: Polyhistidine-tag
sequence annotation: Ochre Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K1709000_sequence/1

LuxI Coding Region
type: SequenceAnnotation
persistent identity: annotation2446990
display id: annotation2446990
version: 1
top level: LuxI-His
location: https://synbiohub.org/public/igem/BBa_K1709000/annotation2446990/range2446990/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1709000/annotation2446990/range2446990/1
type: Range
persistent identity: range2446990
display id: range2446990
version: 1
top level: LuxI-His
direction: direction/forward
start: 1
end: 579
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2446989
display id: annotation2446989
version: 1
top level: LuxI-His
location: https://synbiohub.org/public/igem/BBa_K1709000/annotation2446989/range2446989/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1709000/annotation2446989/range2446989/1
type: Range
persistent identity: range2446989
display id: range2446989
version: 1
top level: LuxI-His
direction: direction/forward
start: 1
end: 3
orientation: inline

Polyhistidine-tag
type: SequenceAnnotation
persistent identity: annotation2446991
display id: annotation2446991
version: 1
top level: LuxI-His
location: https://synbiohub.org/public/igem/BBa_K1709000/annotation2446991/range2446991/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1709000/annotation2446991/range2446991/1
type: Range
persistent identity: range2446991
display id: range2446991
version: 1
top level: LuxI-His
direction: direction/forward
start: 583
end: 606
orientation: inline

Ochre Stop Codon
type: SequenceAnnotation
persistent identity: annotation2446992
display id: annotation2446992
version: 1
top level: LuxI-His
location: https://synbiohub.org/public/igem/BBa_K1709000/annotation2446992/range2446992/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1709000/annotation2446992/range2446992/1
type: Range
persistent identity: range2446992
display id: range2446992
version: 1
top level: LuxI-His
direction: direction/forward
start: 607
end: 609
orientation: inline

BBa_J61104
type: ComponentDefinition
persistent identity: BBa J61104
display id: BBa_J61104
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61104_sequence/1

https://synbiohub.org/public/igem/BBa_K1709006_sequence/1
type: Sequence
persistent identity: BBa K1709006 sequence
display id: BBa_K1709006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaagggacatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaagagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattatccatgcccatcaatgaacagtttaaaaaagcagtcttaaattcacaccatcaccatcaccatgccgcataatactagagaaagaggggacatactagatgaaaaacataaatgccgacgacacatacagaataatcaacaaaatcaaagcttgtagaagcaataatgatatcaatcaatgcttgtctgatatgactaaaatggtccattgcgaatattatttactcgcgatcatttatccacactctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattcaggtgcgccggtgccgtatccggatccgctggaaccgcgtgccgcataatactagagcgcaaaaaaccccgcttcggcggggttttttcgctactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61100_sequence/1
type: Sequence
persistent identity: BBa J61100 sequence
display id: BBa_J61100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1002_sequence/1
type: Sequence
persistent identity: BBa B1002 sequence
display id: BBa_B1002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1709001_sequence/1
type: Sequence
persistent identity: BBa K1709001 sequence
display id: BBa_K1709001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaagggacatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaagagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattatccatgcccatcaatgaacagtttaaaaaagcagtcttaaattcacaccatcaccatcaccatgccgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1709004_sequence/1
type: Sequence
persistent identity: BBa K1709004 sequence
display id: BBa_K1709004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataatcaacaaaatcaaagcttgtagaagcaataatgatatcaatcaatgcttgtctgatatgactaaaatggtccattgcgaatattatttactcgcgatcatttatccacactctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattcaggtgcgccggtgccgtatccggatccgctggaaccgcgtgccgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1709000_sequence/1
type: Sequence
persistent identity: BBa K1709000 sequence
display id: BBa_K1709000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaagagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattatccatgcccatcaatgaacagtttaaaaaagcagtcttaaattcacaccatcaccatcaccatgccgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61104_sequence/1
type: Sequence
persistent identity: BBa J61104 sequence
display id: BBa_J61104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaagggaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z