BBa_K1711000 1 BBa_K1711000 AFB2 auxin-binding F-box protein 2015-09-16T11:00:00Z 2015-09-18T05:34:22Z AFB2 was originally found in the genome of the species Arabidopsis thaliana, a small flowering plant. This part, called AFB2, is an F-box protein that binds the common plant hormone auxin. F-box proteins contain an F-box domain that mediates degradation of other proteins by recognizing substrates for ubiquitination. In our system, a dCas9-degron-repressor complex represses expression of lacZ. In the presence of auxin, the F-box protein and the presence of auxin, the AFB2 binds to auxin and the degron; the F-box protein then recruits the ubiquitin ligase to degrade the dCas9-degron-repressor complex. When this occurs, lacZ is no longer repressed and production of beta galactosidase occurs. Beta galactosidase acts as a reporter for the presence of auxin. false false _2131_ 23387 23387 9 false The parts sequence had to be checked for illegal restriction sites. false Anastasia Nicolov BBa_K1711000_sequence 1 atgaattatttcccagatgaagtaatagagcatgtattcgactttgtaacatctcacaaagacaggaatgctatatctcttgtatgcaaatcatggtacaagattgaaagatacagtaggcaaaaggttttcattggaaactgttatgccattaatccagagaggttgcttcggagattcccatgtctaaagtctttgactttgaaaggaaaacctcattttgcggatttcaatttggttcctcatgaatggggaggttttgtgctaccttggattgaggctttggctagaagccgtgtaggacttgaagagcttaggttgaagaggatggttgttactgatgagagtcttgagctgctttctcgttcttttgtcaattttaagtctttggtccttgttagctgtgaaggttttaccactgatggtcttgcctctattgccgctaattgcaggcatcttcgggatcttgatttgcaagagaatgaaatcgatgatcatagaggtcaatggttaagttgtttcccagacacttgcacgactcttgtcacgctaaactttgcgtgcctcgaaggagaaactaatctggttgctctagagaggcttgttgctaggtctccaaacctaaagagtctgaagctaaatcgtgcagtaccgttagatgcactcgcaaggttaatggcgtgtgcgccgcagatagttgacttaggagtagggtcttatgagaatgacccagattccgagtcttacttgaaactcatggctgtcataaagaaatgcacctcgttgaggagtttgtcgggttttctagaggctgctcctcactgtctctcagctttccacccaatatgtcataacctcacctccttgaatcttagttacgcagctgagattcatggtagccaccttattaagcttattcagcattgcaagaaacttcagcggttatggattttggatagtataggtgacaaagggcttgaagttgtagcttctacatgtaaagagttacaagagcttagggtttttccatctgatttactcggtggaggcaacacagctgtgaccgaagaaggtctagttgccatctcggcaggctgccctaagctccactctatactctacttctgccaacaaatgacaaacgcagctctcgtaaccgttgccaagaactgtccaaatttcatccgtttccgactctgcatcctcgagccaaacaaacccgatcacgtcacatctcaacctctagacgaaggctttggagcaatcgtcaaagcctgcaagagcctgagaaggctttctctctcaggtctccttacagaccaagtcttcctctacatcggaatgtacgcgaatcagctcgagatgctctccatagcctttgcaggagatacagacaaaggcatgctatatgtgttgaatggttgcaaaaagatgaagaaactagagataagggatagtccgtttggggacacggcgcttcttgctgatgtgagcaagtatgaaacaatgcgatccctttggatgtcttcatgtgaagtcacactcagtggatgcaaaaggctcgcagagaaagcgccatggctcaatgtagagatcataaacgagaatgataataaccggatggaagaaaacggacacgaggggaggcagaaagtggataagttgtatctgtaccggactgtggttgggacaagaatggatgcgccgccatttgtgtggattctctaacacccagctttcttgtacaaagtggtgatgggctgca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z