LOCUS       BBa_K1713001            1418 bp    DNA     linear       22-Nov-2024
DEFINITION  Plac/ara-1+strong RBS+LacI(+LVA)+double terminator
ACCESSION   BBa_K1713001
VERSION     BBa_K1713001.1  
FEATURES             Location/Qualifiers
     promoter        1..102
                     /label=Hybrid promoter
     promoter        1..102
                     /label=BBa_K1713000
     misc_feature    115..118
                     /label=conserved
     RBS             111..122
                     /label=BBa_B0034
     misc_feature    1218..1256
                     /label=LVA
     CDS             129..1256
                     /label=lacI-LVA
     misc_feature    129..1256
                     /label=BBa_C0012
     misc_feature    1257..1281
                     /label=Help:Barcodes
     CDS             129..1256
                     /label=BBa_C0012
     stem_loop       1301..1344
                     /label=stem_loop
     misc_feature    1290..1369
                     /label=BBa_B0010
     terminator      1290..1369
                     /label=BBa_B0010
     stem_loop       1385..1404
                     /label=T7 TE
     misc_feature    1411..1411
                     /label=stop
     polyA_site      1405..1418
                     /label=polya
     misc_feature    1378..1418
                     /label=BBa_B0012
     terminator      1378..1418
                     /label=BBa_B0012
     terminator      1290..1418
                     /label=BBa_B0015
ORIGIN
        1 catagcattt ttatccataa gattagcgga tcctaagctt tacaattgtg agcgctcaca
       61 attatgatag attcaattgt gagcggataa caatttcaca catactagag aaagaggaga
      121 aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg
      181 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc
      241 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac
      301 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg
      361 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg
      421 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc
      481 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg
      541 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac
      601 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg
      661 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc
      721 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg
      781 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg
      841 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
      901 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
      961 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
     1021 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
     1081 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc
     1141 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1201 gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct taataactct
     1261 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1321 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1381 cactggctca ccttcgggtg ggcctttctg cgtttata
//