LOCUS       BBa_K1713004             971 bp    DNA     linear       25-Nov-2024
DEFINITION  Plac/ara-1+strong RBS+mRFP+double terminator
ACCESSION   BBa_K1713004
VERSION     BBa_K1713004.1  
FEATURES             Location/Qualifiers
     promoter        1..102
                     /label=Hybrid promoter
     promoter        1..102
                     /label=BBa_K1713000
     misc_feature    115..118
                     /label=conserved
     RBS             111..122
                     /label=BBa_B0034
     CDS             129..803
                     /label=mrfp1
     misc_feature    810..834
                     /label=Help:Barcodes
     CDS             129..834
                     /label=BBa_E1010
     stem_loop       854..897
                     /label=stem_loop
     misc_feature    843..922
                     /label=BBa_B0010
     terminator      843..922
                     /label=BBa_B0010
     stem_loop       938..957
                     /label=T7 TE
     misc_feature    964..964
                     /label=stop
     polyA_site      958..971
                     /label=polya
     misc_feature    931..971
                     /label=BBa_B0012
     terminator      931..971
                     /label=BBa_B0012
     terminator      843..971
                     /label=BBa_B0015
ORIGIN
        1 catagcattt ttatccataa gattagcgga tcctaagctt tacaattgtg agcgctcaca
       61 attatgatag attcaattgt gagcggataa caatttcaca catactagag aaagaggaga
      121 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
      181 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
      241 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
      301 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
      361 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
      421 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
      481 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
      541 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
      601 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
      661 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
      721 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
      781 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
      841 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      901 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      961 ctgcgtttat a
//