LOCUS       BBa_K1713005            2234 bp    DNA     linear       29-May-2024
DEFINITION  Plac/ara-1+strong RBS+araC(+LVA)+double terminator+Plac/ara-1+stron
            g RBS+GFP(+LVA)+double terminator
ACCESSION   BBa_K1713005
VERSION     BBa_K1713005.1  
FEATURES             Location/Qualifiers
     promoter        1..102
                     /label=Hybrid promoter
     promoter        1..102
                     /label=BBa_K1713000
     misc_feature    115..118
                     /label=conserved
     RBS             111..122
                     /label=BBa_B0034
     CDS             129..1004
                     /label=araC
     misc_feature    1005..1037
                     /label=LVA
     misc_feature    1044..1068
                     /label=Help:Barcodes
     CDS             129..1068
                     /label=BBa_C0080
     stem_loop       1088..1131
                     /label=stem_loop
     misc_feature    1077..1156
                     /label=BBa_B0010
     terminator      1077..1156
                     /label=BBa_B0010
     stem_loop       1172..1191
                     /label=T7 TE
     misc_feature    1198..1198
                     /label=stop
     polyA_site      1192..1205
                     /label=polya
     misc_feature    1165..1205
                     /label=BBa_B0012
     terminator      1165..1205
                     /label=BBa_B0012
     terminator      1077..1205
                     /label=BBa_B0015
     misc_feature    1..1205
                     /label=BBa_K1713002
     promoter        1214..1315
                     /label=Hybrid promoter
     promoter        1214..1315
                     /label=BBa_K1713000
     misc_feature    1328..1331
                     /label=conserved
     RBS             1324..1335
                     /label=BBa_B0034
     misc_feature    1342..2061
                     /label=GFP
     misc_feature    2062..2094
                     /label=LVA
     CDS             1342..2097
                     /label=BBa_K082003
     stem_loop       2117..2160
                     /label=stem_loop
     misc_feature    2106..2185
                     /label=BBa_B0010
     terminator      2106..2185
                     /label=BBa_B0010
     stem_loop       2201..2220
                     /label=T7 TE
     misc_feature    2227..2227
                     /label=stop
     polyA_site      2221..2234
                     /label=polya
     misc_feature    2194..2234
                     /label=BBa_B0012
     terminator      2194..2234
                     /label=BBa_B0012
     terminator      2106..2234
                     /label=BBa_B0015
     misc_feature    1214..2234
                     /label=BBa_K1713003
ORIGIN
        1 catagcattt ttatccataa gattagcgga tcctaagctt tacaattgtg agcgctcaca
       61 attatgatag attcaattgt gagcggataa caatttcaca catactagag aaagaggaga
      121 aatactagat ggctgaagcg caaaatgatc ccctgctgcc gggatactcg tttaacgccc
      181 atctggtggc gggtttaacg ccgattgagg ccaatggtta tctcgatttt tttatcgacc
      241 gaccgctggg aatgaaaggt tatattctca atctcaccat tcgcggtcag ggggtggtga
      301 aaaatcaggg acgagaattt gtctgccgac cgggtgatat tttgctgttc ccgccaggag
      361 agattcatca ctacggtcgt catccggagg ctcgcgaatg gtatcaccag tgggtttact
      421 ttcgtccgcg cgcctactgg catgaatggc ttaactggcc gtcaatattt gccaatacgg
      481 gtttctttcg cccggatgaa gcgcaccagc cgcatttcag cgacctgttt gggcaaatca
      541 ttaacgccgg gcaaggggaa gggcgctatt cggagctgct ggcgataaat ctgcttgagc
      601 aattgttact gcggcgcatg gaagcgatta acgagtcgct ccatccaccg atggataatc
      661 gggtacgcga ggcttgtcag tacatcagcg atcacctggc agacagcaat tttgatatcg
      721 ccagcgtcgc acagcatgtt tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc
      781 agttagggat tagcgtctta agctggcgcg aggaccaacg catcagccag gcgaagctgc
      841 ttttgagcac tacccggatg cctatcgcca ccgtcggtcg caatgttggt tttgacgatc
      901 aactctattt ctcgcgagta tttaaaaaat gcaccggggc cagcccgagc gagttccgtg
      961 ccggttgtga agaaaaagtg aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa
     1021 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag
     1081 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1141 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1201 ttatatacta gagcatagca tttttatcca taagattagc ggatcctaag ctttacaatt
     1261 gtgagcgctc acaattatga tagattcaat tgtgagcgga taacaatttc acacatacta
     1321 gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
     1381 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     1441 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     1501 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
     1561 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     1621 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     1681 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     1741 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     1801 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     1861 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     1921 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1981 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     2041 gatgaactat acaaaaggcc tgctgcaaac gacgaaaact acgctttagt agcttaatac
     2101 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2161 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2221 tttctgcgtt tata
//