LOCUS       BBa_K1714006            3218 bp    DNA     linear       23-Nov-2024
DEFINITION  pBad/araC-RBS B0034-Ag43 thanatin 3mer as alpha domain-double 
            terminator
ACCESSION   BBa_K1714006
VERSION     BBa_K1714006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1210
                     /label=BBa_I0500
     misc_feature    1..1079
                     /label=AraC
     promoter        1043..1114
                     /label=PC
     promoter        1080..1210
                     /label=Pbad
     RBS             1219..1230
                     /label=RBS B0034
     misc_feature    1219..1230
                     /label=BBa_B0034
     CDS             1237..1395
                     /label=Ag43 Signal Peptide
     CDS             1402..1612
                     /label=Thanatin 3mer as alpha domain
     CDS             1618..3088
                     /label=Ag43 beta domain
     stem_loop       3089..3218
                     /label=double terminator
     misc_feature    3089..3218
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga aacgacatct gaatacctgc
     1261 tacaggctgg tatggaatca catgacgggc gctttcgtgg ttgcctccga actggcccgc
     1321 gcacggggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcagt cacgtcactc
     1381 ccggtgctgg ctgctagatc cggcagcaaa aaaccggtgc cgattattta ttgcaaccgt
     1441 cgtaccggca aatgccagcg tatgatcaga tccggcagca aaaaaccggt gccgattatt
     1501 tattgcaacc gtcgtaccgg caaatgccag cgtatgatca gatccggcag caaaaaaccg
     1561 gtgccgatta tttattgcaa ccgtcgtacc ggcaaatgcc agcgtatgat cagatctacg
     1621 aatgtcactc tcgcctccgg tgccacctgg aatatccccg ataacgccac ggtgcagtcg
     1681 gtggtggatg acctcagcca tgccggacag attcatttca cctccacccg cacagggaag
     1741 ttcgtaccgg caaccctgaa agtgaaaaac ctgaacggac agaatggcac catcagcctg
     1801 cgtgtacgcc cggatatggc acagaacaat gctgacagac tggtcattga cggcggcagg
     1861 gcaaccggaa aaaccatcct gaacctggtg aacgccggca acagtgcgtc ggggctggcg
     1921 accagcggta agggtattca ggtggtggaa gccattaacg gtgccaccac ggaggaaggg
     1981 gcctttgtcc aggggaacag gctgcaagcc ggtgccttta actactccct caaccgggac
     2041 agtgatgaga gctggtatct gcgcagtgaa aatgcttatc gtgcagaagt ccccctgtat
     2101 gcctccatgc tgacacaggc aatggactat gaccggattg tggcaggctc ccgcagccat
     2161 cagaccggtg taaatggtga aaacaacagc gtccgtctca gcattcaggg cggtcatctc
     2221 ggtcacgata acaatggcgg tattgcccgt ggggccacgc cggaaagcag cggcagctat
     2281 ggattcgtcc gtctggaggg tgacctgatg agaacagagg ttgccggtat gtctgtgacc
     2341 gcgggggtat atggtgctgc tggccattct tccgttgatg ttaaggatga tgacggctcc
     2401 cgtgccggca cggtccggga tgatgccggc agcctgggcg gatacctgaa tctggtacac
     2461 acgtcctccg gcctgtgggc tgacattgtg gcacagggaa cccgccacag catgaaagcg
     2521 tcatcggaca ataacgactt ccgcgcccgg ggctggggct ggctgggctc actggaaacc
     2581 ggtctgccct tcagtatcac tgacaacctg atgctggagc cacaactgca atatacctgg
     2641 cagggacttt ccctggatga cggtaaggac aacgccggtt atgtgaagtt cgggcatggc
     2701 agtgcacaac atgtgcgtgc cggtttccgt ctgggcagcc acaacgatat gacctttggc
     2761 gaaggcacct catcccgtgc ccccctgcgt gacagtgcaa aacacagtgt gagtgaatta
     2821 ccggtgaact ggtgggtaca gccttctgtt atccgcacct tcagctcccg gggagatatg
     2881 cgtgtgggga cttccactgc aagcagcggg atgacgttct ctccctcaca gaatggcaca
     2941 tcactggacc tgcaagccgg actggaagcc cgtgtccggg aaaatatcac cctgggcgtt
     3001 caggccggtt atgcccacag cgtcagcggc agcagcgctg aagggtataa cggtcaggcc
     3061 acactgaatg tgaccttctg atactagagc caggcatcaa ataaaacgaa aggctcagtc
     3121 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     3181 cactggctca ccttcgggtg ggcctttctg cgtttata
//