LOCUS       BBa_K1714007            3290 bp    DNA     linear       26-Nov-2024
DEFINITION  pBad/araC-RBS B0034-Ag43 thanatin 4mer as alpha domain-double 
            terminator
ACCESSION   BBa_K1714007
VERSION     BBa_K1714007.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3290
                     /label=BBa_K1714007
     misc_feature    1..1210
                     /label=BBa_I0500
     misc_feature    1..1079
                     /label=AraC
     promoter        1043..1114
                     /label=PC
     promoter        1080..1210
                     /label=Pbad
     RBS             1219..1230
                     /label=RBS B0034
     CDS             1237..1395
                     /label=Ag43 Signal Peptide
     CDS             1402..1683
                     /label=Thanatin 4mer as alpha domain
     CDS             1684..3160
                     /label=Ag43 beta domain
     stem_loop       3161..3290
                     /label=double terminator
     misc_feature    1219..1230
                     /label=BBa_B0034
     misc_feature    3161..3290
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga aacgacatct gaatacctgc
     1261 tacaggctgg tatggaatca catgacgggc gctttcgtgg ttgcctccga actggcccgc
     1321 gcacggggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcagt cacgtcactc
     1381 ccggtgctgg ctgctagatc cggcagcaaa aaaccggtgc cgattattta ttgcaaccgt
     1441 cgtaccggca aatgccagcg tatgatcaga tccggcagca aaaaaccggt gccgattatt
     1501 tattgcaacc gtcgtaccgg caaatgccag cgtatgatca gatccggcag caaaaaaccg
     1561 gtgccgatta tttattgcaa ccgtcgtacc ggcaaatgcc agcgtatgat cagatccggc
     1621 agcaaaaaac cggtgccgat tatttattgc aaccgtcgta ccggcaaatg ccagcgtatg
     1681 atcagatcta cgaatgtcac tctcgcctcc ggtgccacct ggaatatccc cgataacgcc
     1741 acggtgcagt cggtggtgga tgacctcagc catgccggac agattcattt cacctccacc
     1801 cgcacaggga agttcgtacc ggcaaccctg aaagtgaaaa acctgaacgg acagaatggc
     1861 accatcagcc tgcgtgtacg cccggatatg gcacagaaca atgctgacag actggtcatt
     1921 gacggcggca gggcaaccgg aaaaaccatc ctgaacctgg tgaacgccgg caacagtgcg
     1981 tcggggctgg cgaccagcgg taagggtatt caggtggtgg aagccattaa cggtgccacc
     2041 acggaggaag gggcctttgt ccaggggaac aggctgcaag ccggtgcctt taactactcc
     2101 ctcaaccggg acagtgatga gagctggtat ctgcgcagtg aaaatgctta tcgtgcagaa
     2161 gtccccctgt atgcctccat gctgacacag gcaatggact atgaccggat tgtggcaggc
     2221 tcccgcagcc atcagaccgg tgtaaatggt gaaaacaaca gcgtccgtct cagcattcag
     2281 ggcggtcatc tcggtcacga taacaatggc ggtattgccc gtggggccac gccggaaagc
     2341 agcggcagct atggattcgt ccgtctggag ggtgacctga tgagaacaga ggttgccggt
     2401 atgtctgtga ccgcgggggt atatggtgct gctggccatt cttccgttga tgttaaggat
     2461 gatgacggct cccgtgccgg cacggtccgg gatgatgccg gcagcctggg cggatacctg
     2521 aatctggtac acacgtcctc cggcctgtgg gctgacattg tggcacaggg aacccgccac
     2581 agcatgaaag cgtcatcgga caataacgac ttccgcgccc ggggctgggg ctggctgggc
     2641 tcactggaaa ccggtctgcc cttcagtatc actgacaacc tgatgctgga gccacaactg
     2701 caatatacct ggcagggact ttccctggat gacggtaagg acaacgccgg ttatgtgaag
     2761 ttcgggcatg gcagtgcaca acatgtgcgt gccggtttcc gtctgggcag ccacaacgat
     2821 atgacctttg gcgaaggcac ctcatcccgt gcccccctgc gtgacagtgc aaaacacagt
     2881 gtgagtgaat taccggtgaa ctggtgggta cagccttctg ttatccgcac cttcagctcc
     2941 cggggagata tgcgtgtggg gacttccact gcaagcagcg ggatgacgtt ctctccctca
     3001 cagaatggca catcactgga cctgcaagcc ggactggaag cccgtgtccg ggaaaatatc
     3061 accctgggcg ttcaggccgg ttatgcccac agcgtcagcg gcagcagcgc tgaagggtat
     3121 aacggtcagg ccacactgaa tgtgaccttc tgatactaga gccaggcatc aaataaaacg
     3181 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     3241 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//