BBa_K1717171 1 KERA Protein coding sequence for KeratinaseA 2015-09-12T11:00:00Z 2015-09-18T10:16:33Z Found on the following website: http://www.uniprot.org/uniprot/Q53521. This sequence codes for Keratinase A. It has been optimized for expression in E.coli. false false _2137_ 25516 25516 9 false The bacteria that we are using is a strand of e-coli called K- 12. This strain has a negative granam making it opposite to the gram-positive bacter (Bacillus Licheniformis) the Keratinase that we chose to utilize for our project was found in. After extensive research and looking at the projects of chicago, sheffield and Taiga we learnt that the different ganaam is what causes the issues with the secretion of the keratinase as it gets stuck in the periplasm due to the fact that the signal peptides of the keratin can???t be read by the E. coli. After considering all of this we learned that we must completely remove the gram positive signal sequence, in order to fix this problem. The gram positive signal sequence was completely removed and in its place is PelB (BBa_J32015). false Our Lady of the Snows Catholic Academy 2015 BBa_K1717171_sequence 1 atgaaatacctgctgccgaccgctgcggcaggtctgctgctgctggctgcacaacctgctatggcagctcaacctgcaaagaatgttgagaaggattacatcgttggtttcaaatccggtgtgaagaccgctagcgttaagaaagacatcatcaaagagagcggtggtaaagtggacaaacagttccgtatcatcaacgcggctaaagcgaaactggacaaagaggcgctgaaagaagtcaagaacgacccggacgtcgcttacgtggaagaagaccacgtggcgcacgcactggcgcagaccgtcccgtacggtatcccgctgatcaaagcggacaaagtgcaggctcagggtttcaaaggtgcgaacgtgaaagtggcagtgctggacaccggtatccaggcaagccacccagacctgaacgtggtgggtggcgcttcttttgttgctggtgaagcatacaacaccgatggtaacggtcacggcactcacgtggcaggcactgttgctgcactggataatactacgggtgtactgggtgttgcaccaagcgtatctctgtacgcagttaaagtactgaacagcagcggcagcggtagctacagcggtatcgtaagcggtatcgaatgggcaaccaccaacggtatggatgttattaacatgtccctgggcggcgcctccggttccactgctatgaaacaggccgttgataacgcctacgctcgtggtgttgtcgtcgtagcggcagccggtaactccggctcttccggcaatactaacaccattggctacccggctaaatacgattccgtaattgccgttggcgcggttgattccaactccaaccgcgcgtccttctcctctgtaggcgctgaactggaagtaatggctccgggcgcgggcgtttattctacttatccgacgaacacctatgcgaccctgaacggcacctctatggtttctccgcatgttgcgggcgcggcggccctgattctgtctaaacatccgaatctgtctgcctctcaggttcgtaaccgcctgtcttctacggcgacctatctgggctcttctttttattatggcaaaggcctgattaacgttgaagccgccgcgcagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z