BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898429
1
modified thr terminator
range1898429
1
10
31
annotation1898431
1
PolyA
range1898431
1
1
9
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898428
1
B1006
range1898428
1
1
39
BBa_K081005
1
BBa_K081005
constitutive promoter family member and RBS
2008-10-17T11:00:00Z
2015-05-08T01:08:34Z
Promoter: John Anderson.
RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Released HQ 2013
Constitutive promoter (strong) with RBS (strong, efficiency=0.3)
false
true
_227_
0
2583
9
In stock
true
We used BioBrick Standard Assembly.
true
Lorenzo Pasotti, Paolo Magni
component1981863
1
BBa_J23100
component1981865
1
BBa_B0030
annotation1981863
1
BBa_J23100
range1981863
1
1
35
annotation1981865
1
BBa_B0030
range1981865
1
44
58
BBa_K1719000
1
BBa_K1719000
A gene encoding leucine dehydrogenase
2015-09-04T11:00:00Z
2015-09-17T05:26:13Z
asdasdasd
asdasdasdasd
false
false
_2139_
25238
25236
9
false
asdasdasdasd
false
Lin Shi
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1719006
1
BBa_K1719006
Promoter+RBS+LeuDH+Terminator
2015-09-04T11:00:00Z
2015-09-17T04:43:39Z
aassvvbb
aassvvbb
false
false
_2139_
25238
25238
9
false
aassvvbb
false
Shibo Liu
component2443881
1
BBa_B1006
component2443875
1
BBa_K081005
component2443876
1
BBa_K1719000
annotation2443875
1
BBa_K081005
range2443875
1
1
58
annotation2443881
1
BBa_B1006
range2443881
1
1174
1212
annotation2443876
1
BBa_K1719000
range2443876
1
65
1165
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K081005_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_K1719000_sequence
1
atgacattggaaatcttcgaatatctggaaaagtatgattatgaacaggttgtgttttgtcaggacaaagagagcggactgaaagcaattatcgccattcatgacactaccctgggaccggctctcggcggtacccgcatgtggacctatgattcagaggaagcggcaatcgaagatgcactccgtctggcaaaaggcatgacatataaaaacgcagctgctggtttaaatctgggtggcgcgaaaaccgtcattattggcgatccgcgtaaagataaatcagaagcgatgtttcgtgccctgggtcgctacatccaagggctgaacgggcgttatattaccgcggaagatgtaggcactacggttgacgatatggatatcattcacgaggagactgattttgtcacgggcatctctccgtccttcgggagctccggaaatccgtctccggttaccgcttatggggtttaccgcgggatgaaagcggcggcgaaagaggcgtttggtaccgacaatttagagggcaaagtgattgcggtgcaaggtgtgggcaatgtggcctatcacctttgcaagcacctgcacgccgagggtgcaaagttaattgtcaccgacatcaataaggaggccgtccagcgtgccgtagaggagttcggtgctacggcggtggagcctaacgaaatttatggggtggaatgcgacatttatgcgccatgtgcactcggcgcaacggtaaatgatgaaaccatcccacagcttaaagcgaaagtgattgccggcagcgctaataatcagcttaaagaagatcggcatggcgatatcattcatgaaatgggcatcgtctacgccccggattatgttattaatgcgggtggtgttattaacgtggcggatgaactgtacggctataaccgcgaacgtgcactgaaacgtgtggaaagcatttacgacaccattgccaaggtgatcgaaatctcgaaacgggatggcatcgccacgtacgtagccgcggatcggttggcggaagaacgcatcgcctcgttgaaaaacagtcgctcgacgtatctgcgcaacggccatgacatcattagtcgccggtaa
BBa_K1719006_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgacattggaaatcttcgaatatctggaaaagtatgattatgaacaggttgtgttttgtcaggacaaagagagcggactgaaagcaattatcgccattcatgacactaccctgggaccggctctcggcggtacccgcatgtggacctatgattcagaggaagcggcaatcgaagatgcactccgtctggcaaaaggcatgacatataaaaacgcagctgctggtttaaatctgggtggcgcgaaaaccgtcattattggcgatccgcgtaaagataaatcagaagcgatgtttcgtgccctgggtcgctacatccaagggctgaacgggcgttatattaccgcggaagatgtaggcactacggttgacgatatggatatcattcacgaggagactgattttgtcacgggcatctctccgtccttcgggagctccggaaatccgtctccggttaccgcttatggggtttaccgcgggatgaaagcggcggcgaaagaggcgtttggtaccgacaatttagagggcaaagtgattgcggtgcaaggtgtgggcaatgtggcctatcacctttgcaagcacctgcacgccgagggtgcaaagttaattgtcaccgacatcaataaggaggccgtccagcgtgccgtagaggagttcggtgctacggcggtggagcctaacgaaatttatggggtggaatgcgacatttatgcgccatgtgcactcggcgcaacggtaaatgatgaaaccatcccacagcttaaagcgaaagtgattgccggcagcgctaataatcagcttaaagaagatcggcatggcgatatcattcatgaaatgggcatcgtctacgccccggattatgttattaatgcgggtggtgttattaacgtggcggatgaactgtacggctataaccgcgaacgtgcactgaaacgtgtggaaagcatttacgacaccattgccaaggtgatcgaaatctcgaaacgggatggcatcgccacgtacgtagccgcggatcggttggcggaagaacgcatcgcctcgttgaaaaacagtcgctcgacgtatctgcgcaacggccatgacatcattagtcgccggtaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z