LOCUS       BBa_K1719007            1458 bp    DNA     linear       17-May-2024
DEFINITION  Promoter+RBS+FDH+Terminator
ACCESSION   BBa_K1719007
VERSION     BBa_K1719007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1321
                     /label=BBa_K1719001
     stem_loop       1341..1384
                     /label=stem_loop
     misc_feature    1330..1409
                     /label=BBa_B0010
     terminator      1330..1409
                     /label=BBa_B0010
     stem_loop       1425..1444
                     /label=T7 TE
     misc_feature    1451..1451
                     /label=stop
     polyA_site      1445..1458
                     /label=polya
     misc_feature    1418..1458
                     /label=BBa_B0012
     terminator      1418..1458
                     /label=BBa_B0012
     terminator      1330..1458
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaattgtcct
      241 ggtcctgtat gacgcgggca aacatgcggc cgatgaggag aaactttacg gatctacgga
      301 aaataaactg gggatcgcca attggctgaa agatcagggc cacgaactga tcaccacaag
      361 tgataaagaa ggggaaacaa gcgaattgga taagcatatt ccggatgcag atatcattat
      421 tactacgccg tttcatccag catatatcac caaagaacgc ctcgataaag ctaagaacct
      481 gaagttggtg gtagtcgcag gggtggggtc ggatcatatt gacctggatt acattaatca
      541 gaccgggaaa aaaatttctg tgttagaagt taccggcagt aatgtcgttt ctgtggccga
      601 acacgtggtt atgaccatgt tggttctggt gcgcaacttt gtgccagcac atgaacagat
      661 tatcaatcac gactgggagg ttgccgcgat cgcaaaagac gcctacgata tcgaaggaaa
      721 aactatcgct actatcggtg cgggccgcat cggttatcgt gttttggagc gtcttctgcc
      781 ttttaacccg aaagagctct tatattacga ttatcaggcc ttaccgaaag aagcggaaga
      841 gaaagtaggt gcgcgtcgtg tggaaaatat cgaagaatta gtagcgcaag cagatatcgt
      901 gacggtgaac gcgcctctcc atgccggtac gaaaggcctg attaataagg aactcctgtc
      961 caaattcaaa aaaggtgcgt ggcttgtgaa taccgctcgc ggtgcgattt gcgtcgctga
     1021 agacgtggcg gcagcgctgg agagcggcca acttcgcggt tatggcggtg acgtatggtt
     1081 tccgcagccg gctccgaaag accacccatg gcgcgacatg cgtaacaaat atggcgcggg
     1141 caacgccatg accccgcatt attcgggtac caccctggat gcccaaaccc ggtacgcaga
     1201 gggcaccaag aatattctgg agtcattttt cacgggcaaa ttcgattatc ggccgcagga
     1261 tattattctg ttgaacggag agtatgttac gaaggcctat ggcaaacacg ataaaaagta
     1321 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1381 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1441 ggcctttctg cgtttata
//