LOCUS       BBa_K1719008            1464 bp    DNA     linear       22-Nov-2024
DEFINITION  LacI+B0034+LeuDH+B0015
ACCESSION   BBa_K1719008
VERSION     BBa_K1719008.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1327
                     /label=BBa_K1719000
     stem_loop       1347..1390
                     /label=stem_loop
     misc_feature    1336..1415
                     /label=BBa_B0010
     terminator      1336..1415
                     /label=BBa_B0010
     stem_loop       1431..1450
                     /label=T7 TE
     misc_feature    1457..1457
                     /label=stop
     polyA_site      1451..1464
                     /label=polya
     misc_feature    1424..1464
                     /label=BBa_B0012
     terminator      1424..1464
                     /label=BBa_B0012
     terminator      1336..1464
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga cattggaaat
      241 cttcgaatat ctggaaaagt atgattatga acaggttgtg ttttgtcagg acaaagagag
      301 cggactgaaa gcaattatcg ccattcatga cactaccctg ggaccggctc tcggcggtac
      361 ccgcatgtgg acctatgatt cagaggaagc ggcaatcgaa gatgcactcc gtctggcaaa
      421 aggcatgaca tataaaaacg cagctgctgg tttaaatctg ggtggcgcga aaaccgtcat
      481 tattggcgat ccgcgtaaag ataaatcaga agcgatgttt cgtgccctgg gtcgctacat
      541 ccaagggctg aacgggcgtt atattaccgc ggaagatgta ggcactacgg ttgacgatat
      601 ggatatcatt cacgaggaga ctgattttgt cacgggcatc tctccgtcct tcgggagctc
      661 cggaaatccg tctccggtta ccgcttatgg ggtttaccgc gggatgaaag cggcggcgaa
      721 agaggcgttt ggtaccgaca atttagaggg caaagtgatt gcggtgcaag gtgtgggcaa
      781 tgtggcctat cacctttgca agcacctgca cgccgagggt gcaaagttaa ttgtcaccga
      841 catcaataag gaggccgtcc agcgtgccgt agaggagttc ggtgctacgg cggtggagcc
      901 taacgaaatt tatggggtgg aatgcgacat ttatgcgcca tgtgcactcg gcgcaacggt
      961 aaatgatgaa accatcccac agcttaaagc gaaagtgatt gccggcagcg ctaataatca
     1021 gcttaaagaa gatcggcatg gcgatatcat tcatgaaatg ggcatcgtct acgccccgga
     1081 ttatgttatt aatgcgggtg gtgttattaa cgtggcggat gaactgtacg gctataaccg
     1141 cgaacgtgca ctgaaacgtg tggaaagcat ttacgacacc attgccaagg tgatcgaaat
     1201 ctcgaaacgg gatggcatcg ccacgtacgt agccgcggat cggttggcgg aagaacgcat
     1261 cgcctcgttg aaaaacagtc gctcgacgta tctgcgcaac ggccatgaca tcattagtcg
     1321 ccggtaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1381 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1441 cgggtgggcc tttctgcgtt tata
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