LOCUS BBa_K1719009 2930 bp DNA linear 22-Dec-2024 DEFINITION Circuit encoding leucine dehydrogenase and formate dehydrogenase ACCESSION BBa_K1719009 VERSION BBa_K1719009.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 CDS 227..1321 /label=BBa_K1719001 stem_loop 1341..1384 /label=stem_loop misc_feature 1330..1409 /label=BBa_B0010 terminator 1330..1409 /label=BBa_B0010 stem_loop 1425..1444 /label=T7 TE misc_feature 1451..1451 /label=stop polyA_site 1445..1458 /label=polya misc_feature 1418..1458 /label=BBa_B0012 terminator 1418..1458 /label=BBa_B0012 terminator 1330..1458 /label=BBa_B0015 misc_feature 1..1458 /label=BBa_K1719007 CDS 1467..1554 /label=end of LacI coding region (inactive) misc_feature 1467..1666 /label=BBa_R0010 misc_feature 1555..1592 /label=CAP binding site promoter 1603..1608 /label=-35 promoter 1627..1632 /label=-10 misc_feature 1632..1666 /label=LacI binding site misc_feature 1639..1639 /label=start promoter 1467..1666 /label=BBa_R0010 misc_feature 1679..1682 /label=conserved RBS 1675..1686 /label=BBa_B0034 CDS 1693..2793 /label=BBa_K1719000 stem_loop 2813..2856 /label=stem_loop misc_feature 2802..2881 /label=BBa_B0010 terminator 2802..2881 /label=BBa_B0010 stem_loop 2897..2916 /label=T7 TE misc_feature 2923..2923 /label=stop polyA_site 2917..2930 /label=polya misc_feature 2890..2930 /label=BBa_B0012 terminator 2890..2930 /label=BBa_B0012 terminator 2802..2930 /label=BBa_B0015 misc_feature 1467..2930 /label=BBa_K1719008 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaattgtcct 241 ggtcctgtat gacgcgggca aacatgcggc cgatgaggag aaactttacg gatctacgga 301 aaataaactg gggatcgcca attggctgaa agatcagggc cacgaactga tcaccacaag 361 tgataaagaa ggggaaacaa gcgaattgga taagcatatt ccggatgcag atatcattat 421 tactacgccg tttcatccag catatatcac caaagaacgc ctcgataaag ctaagaacct 481 gaagttggtg gtagtcgcag gggtggggtc ggatcatatt gacctggatt acattaatca 541 gaccgggaaa aaaatttctg tgttagaagt taccggcagt aatgtcgttt ctgtggccga 601 acacgtggtt atgaccatgt tggttctggt gcgcaacttt gtgccagcac atgaacagat 661 tatcaatcac gactgggagg ttgccgcgat cgcaaaagac gcctacgata tcgaaggaaa 721 aactatcgct actatcggtg cgggccgcat cggttatcgt gttttggagc gtcttctgcc 781 ttttaacccg aaagagctct tatattacga ttatcaggcc ttaccgaaag aagcggaaga 841 gaaagtaggt gcgcgtcgtg tggaaaatat cgaagaatta gtagcgcaag cagatatcgt 901 gacggtgaac gcgcctctcc atgccggtac gaaaggcctg attaataagg aactcctgtc 961 caaattcaaa aaaggtgcgt ggcttgtgaa taccgctcgc ggtgcgattt gcgtcgctga 1021 agacgtggcg gcagcgctgg agagcggcca acttcgcggt tatggcggtg acgtatggtt 1081 tccgcagccg gctccgaaag accacccatg gcgcgacatg cgtaacaaat atggcgcggg 1141 caacgccatg accccgcatt attcgggtac caccctggat gcccaaaccc ggtacgcaga 1201 gggcaccaag aatattctgg agtcattttt cacgggcaaa ttcgattatc ggccgcagga 1261 tattattctg ttgaacggag agtatgttac gaaggcctat ggcaaacacg ataaaaagta 1321 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 1381 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 1441 ggcctttctg cgtttatata ctagagcaat acgcaaaccg cctctccccg cgcgttggcc 1501 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 1561 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 1621 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag 1681 gagaaatact agatgacatt ggaaatcttc gaatatctgg aaaagtatga ttatgaacag 1741 gttgtgtttt gtcaggacaa agagagcgga ctgaaagcaa ttatcgccat tcatgacact 1801 accctgggac cggctctcgg cggtacccgc atgtggacct atgattcaga ggaagcggca 1861 atcgaagatg cactccgtct ggcaaaaggc atgacatata aaaacgcagc tgctggttta 1921 aatctgggtg gcgcgaaaac cgtcattatt ggcgatccgc gtaaagataa atcagaagcg 1981 atgtttcgtg ccctgggtcg ctacatccaa gggctgaacg ggcgttatat taccgcggaa 2041 gatgtaggca ctacggttga cgatatggat atcattcacg aggagactga ttttgtcacg 2101 ggcatctctc cgtccttcgg gagctccgga aatccgtctc cggttaccgc ttatggggtt 2161 taccgcggga tgaaagcggc ggcgaaagag gcgtttggta ccgacaattt agagggcaaa 2221 gtgattgcgg tgcaaggtgt gggcaatgtg gcctatcacc tttgcaagca cctgcacgcc 2281 gagggtgcaa agttaattgt caccgacatc aataaggagg ccgtccagcg tgccgtagag 2341 gagttcggtg ctacggcggt ggagcctaac gaaatttatg gggtggaatg cgacatttat 2401 gcgccatgtg cactcggcgc aacggtaaat gatgaaacca tcccacagct taaagcgaaa 2461 gtgattgccg gcagcgctaa taatcagctt aaagaagatc ggcatggcga tatcattcat 2521 gaaatgggca tcgtctacgc cccggattat gttattaatg cgggtggtgt tattaacgtg 2581 gcggatgaac tgtacggcta taaccgcgaa cgtgcactga aacgtgtgga aagcatttac 2641 gacaccattg ccaaggtgat cgaaatctcg aaacgggatg gcatcgccac gtacgtagcc 2701 gcggatcggt tggcggaaga acgcatcgcc tcgttgaaaa acagtcgctc gacgtatctg 2761 cgcaacggcc atgacatcat tagtcgccgg taatactaga gccaggcatc aaataaaacg 2821 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 2881 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //