LOCUS       BBa_K1719010            2678 bp    DNA     linear       26-Dec-2024
DEFINITION  Circuit encoding leucine dehydrogenase and formate dehydrogenase
ACCESSION   BBa_K1719010
VERSION     BBa_K1719010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    1..58
                     /label=BBa_K081005
     CDS             65..1165
                     /label=BBa_K1719000
     misc_feature    1174..1212
                     /label=B1006
     stem_loop       1183..1204
                     /label=modified thr terminator
     polyA_site      1205..1212
                     /label=PolyA
     polyA_site      1174..1182
                     /label=PolyA
     terminator      1174..1212
                     /label=BBa_B1006
     misc_feature    1..1212
                     /label=BBa_K1719006
     CDS             1221..1308
                     /label=end of LacI coding region (inactive)
     misc_feature    1221..1420
                     /label=BBa_R0010
     misc_feature    1309..1346
                     /label=CAP binding site
     promoter        1357..1362
                     /label=-35
     promoter        1381..1386
                     /label=-10
     misc_feature    1386..1420
                     /label=LacI binding site
     misc_feature    1393..1393
                     /label=start
     promoter        1221..1420
                     /label=BBa_R0010
     misc_feature    1433..1436
                     /label=conserved
     RBS             1429..1440
                     /label=BBa_B0034
     misc_feature    1221..1440
                     /label=BBa_J04500
     CDS             1447..2541
                     /label=BBa_K1719001
     stem_loop       2561..2604
                     /label=stem_loop
     misc_feature    2550..2629
                     /label=BBa_B0010
     terminator      2550..2629
                     /label=BBa_B0010
     stem_loop       2645..2664
                     /label=T7 TE
     misc_feature    2671..2671
                     /label=stop
     polyA_site      2665..2678
                     /label=polya
     misc_feature    2638..2678
                     /label=BBa_B0012
     terminator      2638..2678
                     /label=BBa_B0012
     terminator      2550..2678
                     /label=BBa_B0015
     misc_feature    1221..2678
                     /label=BBa_K1719007
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatgaca ttggaaatct tcgaatatct ggaaaagtat gattatgaac aggttgtgtt
      121 ttgtcaggac aaagagagcg gactgaaagc aattatcgcc attcatgaca ctaccctggg
      181 accggctctc ggcggtaccc gcatgtggac ctatgattca gaggaagcgg caatcgaaga
      241 tgcactccgt ctggcaaaag gcatgacata taaaaacgca gctgctggtt taaatctggg
      301 tggcgcgaaa accgtcatta ttggcgatcc gcgtaaagat aaatcagaag cgatgtttcg
      361 tgccctgggt cgctacatcc aagggctgaa cgggcgttat attaccgcgg aagatgtagg
      421 cactacggtt gacgatatgg atatcattca cgaggagact gattttgtca cgggcatctc
      481 tccgtccttc gggagctccg gaaatccgtc tccggttacc gcttatgggg tttaccgcgg
      541 gatgaaagcg gcggcgaaag aggcgtttgg taccgacaat ttagagggca aagtgattgc
      601 ggtgcaaggt gtgggcaatg tggcctatca cctttgcaag cacctgcacg ccgagggtgc
      661 aaagttaatt gtcaccgaca tcaataagga ggccgtccag cgtgccgtag aggagttcgg
      721 tgctacggcg gtggagccta acgaaattta tggggtggaa tgcgacattt atgcgccatg
      781 tgcactcggc gcaacggtaa atgatgaaac catcccacag cttaaagcga aagtgattgc
      841 cggcagcgct aataatcagc ttaaagaaga tcggcatggc gatatcattc atgaaatggg
      901 catcgtctac gccccggatt atgttattaa tgcgggtggt gttattaacg tggcggatga
      961 actgtacggc tataaccgcg aacgtgcact gaaacgtgtg gaaagcattt acgacaccat
     1021 tgccaaggtg atcgaaatct cgaaacggga tggcatcgcc acgtacgtag ccgcggatcg
     1081 gttggcggaa gaacgcatcg cctcgttgaa aaacagtcgc tcgacgtatc tgcgcaacgg
     1141 ccatgacatc attagtcgcc ggtaatacta gagaaaaaaa aaccccgccc ctgacagggc
     1201 ggggtttttt tttactagag caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1261 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1321 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1381 tatgttgtgt ggaattgtga gcggataaca atttcacaca tactagagaa agaggagaaa
     1441 tactagatga aaattgtcct ggtcctgtat gacgcgggca aacatgcggc cgatgaggag
     1501 aaactttacg gatctacgga aaataaactg gggatcgcca attggctgaa agatcagggc
     1561 cacgaactga tcaccacaag tgataaagaa ggggaaacaa gcgaattgga taagcatatt
     1621 ccggatgcag atatcattat tactacgccg tttcatccag catatatcac caaagaacgc
     1681 ctcgataaag ctaagaacct gaagttggtg gtagtcgcag gggtggggtc ggatcatatt
     1741 gacctggatt acattaatca gaccgggaaa aaaatttctg tgttagaagt taccggcagt
     1801 aatgtcgttt ctgtggccga acacgtggtt atgaccatgt tggttctggt gcgcaacttt
     1861 gtgccagcac atgaacagat tatcaatcac gactgggagg ttgccgcgat cgcaaaagac
     1921 gcctacgata tcgaaggaaa aactatcgct actatcggtg cgggccgcat cggttatcgt
     1981 gttttggagc gtcttctgcc ttttaacccg aaagagctct tatattacga ttatcaggcc
     2041 ttaccgaaag aagcggaaga gaaagtaggt gcgcgtcgtg tggaaaatat cgaagaatta
     2101 gtagcgcaag cagatatcgt gacggtgaac gcgcctctcc atgccggtac gaaaggcctg
     2161 attaataagg aactcctgtc caaattcaaa aaaggtgcgt ggcttgtgaa taccgctcgc
     2221 ggtgcgattt gcgtcgctga agacgtggcg gcagcgctgg agagcggcca acttcgcggt
     2281 tatggcggtg acgtatggtt tccgcagccg gctccgaaag accacccatg gcgcgacatg
     2341 cgtaacaaat atggcgcggg caacgccatg accccgcatt attcgggtac caccctggat
     2401 gcccaaaccc ggtacgcaga gggcaccaag aatattctgg agtcattttt cacgggcaaa
     2461 ttcgattatc ggccgcagga tattattctg ttgaacggag agtatgttac gaaggcctat
     2521 ggcaaacacg ataaaaagta atactagagc caggcatcaa ataaaacgaa aggctcagtc
     2581 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2641 cactggctca ccttcgggtg ggcctttctg cgtttata
//