##gff-version 3
##sequence-region BBa_K1719010 1 2678
BBa_K1719010	.	engineered_region	1221	2678	.	+	0	ID=BBa_K1719007;Name=BBa_K1719007
BBa_K1719010	.	CDS	1447	2541	.	+	0	ID=BBa_K1719001;Name=BBa_K1719001;Parent=BBa_K1719007
BBa_K1719010	.	terminator	2550	2678	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1719007
BBa_K1719010	.	terminator	2638	2678	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1719010	.	stem_loop	2645	2664	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1719010	.	polyA_site	2665	2678	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1719010	.	stop_codon	2671	2671	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1719010	.	engineered_region	2638	2678	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1719010	.	terminator	2550	2629	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1719010	.	stem_loop	2561	2604	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1719010	.	engineered_region	2550	2629	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1719010	.	engineered_region	1221	1440	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1719007
BBa_K1719010	.	promoter	1221	1420	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1719010	.	CDS	1221	1308	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1719010	.	promoter	1381	1386	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1719010	.	non_covalent_binding_site	1386	1420	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1719010	.	engineered_region	1221	1420	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1719010	.	promoter	1357	1362	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1719010	.	non_covalent_binding_site	1309	1346	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1719010	.	start_codon	1393	1393	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1719010	.	ribosome_entry_site	1429	1440	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1719010	.	conserved_region	1433	1436	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1719010	.	engineered_region	1	1212	.	+	0	ID=BBa_K1719006;Name=BBa_K1719006
BBa_K1719010	.	engineered_region	1	58	.	+	0	ID=BBa_K081005;Name=BBa_K081005;Parent=BBa_K1719006
BBa_K1719010	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K1719010	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1719010	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1719010	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1719010	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
BBa_K1719010	.	CDS	65	1165	.	+	0	ID=BBa_K1719000;Name=BBa_K1719000;Parent=BBa_K1719006
BBa_K1719010	.	terminator	1174	1212	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K1719006
BBa_K1719010	.	polyA_site	1174	1182	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1719010	.	polyA_site	1205	1212	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1719010	.	engineered_region	1174	1212	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1719010	.	stem_loop	1183	1204	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
>BBa_K1719010
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgacattggaaatc
ttcgaatatctggaaaagtatgattatgaacaggttgtgttttgtcaggacaaagagagcggactgaaagcaattatcg
ccattcatgacactaccctgggaccggctctcggcggtacccgcatgtggacctatgattcagaggaagcggcaatcga
agatgcactccgtctggcaaaaggcatgacatataaaaacgcagctgctggtttaaatctgggtggcgcgaaaaccgtc
attattggcgatccgcgtaaagataaatcagaagcgatgtttcgtgccctgggtcgctacatccaagggctgaacgggc
gttatattaccgcggaagatgtaggcactacggttgacgatatggatatcattcacgaggagactgattttgtcacggg
catctctccgtccttcgggagctccggaaatccgtctccggttaccgcttatggggtttaccgcgggatgaaagcggcg
gcgaaagaggcgtttggtaccgacaatttagagggcaaagtgattgcggtgcaaggtgtgggcaatgtggcctatcacc
tttgcaagcacctgcacgccgagggtgcaaagttaattgtcaccgacatcaataaggaggccgtccagcgtgccgtaga
ggagttcggtgctacggcggtggagcctaacgaaatttatggggtggaatgcgacatttatgcgccatgtgcactcggc
gcaacggtaaatgatgaaaccatcccacagcttaaagcgaaagtgattgccggcagcgctaataatcagcttaaagaag
atcggcatggcgatatcattcatgaaatgggcatcgtctacgccccggattatgttattaatgcgggtggtgttattaa
cgtggcggatgaactgtacggctataaccgcgaacgtgcactgaaacgtgtggaaagcatttacgacaccattgccaag
gtgatcgaaatctcgaaacgggatggcatcgccacgtacgtagccgcggatcggttggcggaagaacgcatcgcctcgt
tgaaaaacagtcgctcgacgtatctgcgcaacggccatgacatcattagtcgccggtaatactagagaaaaaaaaaccc
cgcccctgacagggcggggtttttttttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcatt
aggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacata
ctagagaaagaggagaaatactagatgaaaattgtcctggtcctgtatgacgcgggcaaacatgcggccgatgaggaga
aactttacggatctacggaaaataaactggggatcgccaattggctgaaagatcagggccacgaactgatcaccacaag
tgataaagaaggggaaacaagcgaattggataagcatattccggatgcagatatcattattactacgccgtttcatcca
gcatatatcaccaaagaacgcctcgataaagctaagaacctgaagttggtggtagtcgcaggggtggggtcggatcata
ttgacctggattacattaatcagaccgggaaaaaaatttctgtgttagaagttaccggcagtaatgtcgtttctgtggc
cgaacacgtggttatgaccatgttggttctggtgcgcaactttgtgccagcacatgaacagattatcaatcacgactgg
gaggttgccgcgatcgcaaaagacgcctacgatatcgaaggaaaaactatcgctactatcggtgcgggccgcatcggtt
atcgtgttttggagcgtcttctgccttttaacccgaaagagctcttatattacgattatcaggccttaccgaaagaagc
ggaagagaaagtaggtgcgcgtcgtgtggaaaatatcgaagaattagtagcgcaagcagatatcgtgacggtgaacgcg
cctctccatgccggtacgaaaggcctgattaataaggaactcctgtccaaattcaaaaaaggtgcgtggcttgtgaata
ccgctcgcggtgcgatttgcgtcgctgaagacgtggcggcagcgctggagagcggccaacttcgcggttatggcggtga
cgtatggtttccgcagccggctccgaaagaccacccatggcgcgacatgcgtaacaaatatggcgcgggcaacgccatg
accccgcattattcgggtaccaccctggatgcccaaacccggtacgcagagggcaccaagaatattctggagtcatttt
tcacgggcaaattcgattatcggccgcaggatattattctgttgaacggagagtatgttacgaaggcctatggcaaaca
cgataaaaagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata