BBa_K1719002 1 BBa_K1719002 leucine dehydrogenase + Terminator 2015-09-04T11:00:00Z 2015-09-17T01:50:04Z asdasd asdasdasd false false _2139_ 25238 25236 9 false asdasd false Lin Shi component2443828 1 BBa_K1719000 component2443833 1 BBa_B1006 annotation2443833 1 BBa_B1006 range2443833 1 1110 1148 annotation2443828 1 BBa_K1719000 range2443828 1 1 1101 BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898431 1 PolyA range1898431 1 1 9 annotation1898430 1 PolyA range1898430 1 32 39 annotation1898428 1 B1006 range1898428 1 1 39 annotation1898429 1 modified thr terminator range1898429 1 10 31 BBa_K1719011 1 BBa_K1719011 Promoter and RBS 2015-09-04T11:00:00Z 2015-09-16T02:38:07Z aassbbnn aassbbn false false _2139_ 25238 25238 9 false aasdbn false Shibo Liu component2444075 1 BBa_R0010 component2444083 1 BBa_B0032 annotation2444075 1 BBa_R0010 range2444075 1 1 200 annotation2444083 1 BBa_B0032 range2444083 1 209 221 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961227 1 start range1961227 1 173 173 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_K1719000 1 BBa_K1719000 A gene encoding leucine dehydrogenase 2015-09-04T11:00:00Z 2015-09-17T05:26:13Z asdasdasd asdasdasdasd false false _2139_ 25238 25236 9 false asdasdasdasd false Lin Shi BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 BBa_K1719012 1 BBa_K1719012 LacI+B0032+leudh+B1006 2015-09-04T11:00:00Z 2015-09-17T04:40:55Z iGEM by Wang Feng false false _2139_ 25238 25230 9 false produce LeuDH false Feng Wang component2444102 1 BBa_K1719002 component2444095 1 BBa_K1719011 annotation2444095 1 BBa_K1719011 range2444095 1 1 221 annotation2444102 1 BBa_K1719002 range2444102 1 228 1375 BBa_K1719002_sequence 1 atgacattggaaatcttcgaatatctggaaaagtatgattatgaacaggttgtgttttgtcaggacaaagagagcggactgaaagcaattatcgccattcatgacactaccctgggaccggctctcggcggtacccgcatgtggacctatgattcagaggaagcggcaatcgaagatgcactccgtctggcaaaaggcatgacatataaaaacgcagctgctggtttaaatctgggtggcgcgaaaaccgtcattattggcgatccgcgtaaagataaatcagaagcgatgtttcgtgccctgggtcgctacatccaagggctgaacgggcgttatattaccgcggaagatgtaggcactacggttgacgatatggatatcattcacgaggagactgattttgtcacgggcatctctccgtccttcgggagctccggaaatccgtctccggttaccgcttatggggtttaccgcgggatgaaagcggcggcgaaagaggcgtttggtaccgacaatttagagggcaaagtgattgcggtgcaaggtgtgggcaatgtggcctatcacctttgcaagcacctgcacgccgagggtgcaaagttaattgtcaccgacatcaataaggaggccgtccagcgtgccgtagaggagttcggtgctacggcggtggagcctaacgaaatttatggggtggaatgcgacatttatgcgccatgtgcactcggcgcaacggtaaatgatgaaaccatcccacagcttaaagcgaaagtgattgccggcagcgctaataatcagcttaaagaagatcggcatggcgatatcattcatgaaatgggcatcgtctacgccccggattatgttattaatgcgggtggtgttattaacgtggcggatgaactgtacggctataaccgcgaacgtgcactgaaacgtgtggaaagcatttacgacaccattgccaaggtgatcgaaatctcgaaacgggatggcatcgccacgtacgtagccgcggatcggttggcggaagaacgcatcgcctcgttgaaaaacagtcgctcgacgtatctgcgcaacggccatgacatcattagtcgccggtaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K1719012_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgacattggaaatcttcgaatatctggaaaagtatgattatgaacaggttgtgttttgtcaggacaaagagagcggactgaaagcaattatcgccattcatgacactaccctgggaccggctctcggcggtacccgcatgtggacctatgattcagaggaagcggcaatcgaagatgcactccgtctggcaaaaggcatgacatataaaaacgcagctgctggtttaaatctgggtggcgcgaaaaccgtcattattggcgatccgcgtaaagataaatcagaagcgatgtttcgtgccctgggtcgctacatccaagggctgaacgggcgttatattaccgcggaagatgtaggcactacggttgacgatatggatatcattcacgaggagactgattttgtcacgggcatctctccgtccttcgggagctccggaaatccgtctccggttaccgcttatggggtttaccgcgggatgaaagcggcggcgaaagaggcgtttggtaccgacaatttagagggcaaagtgattgcggtgcaaggtgtgggcaatgtggcctatcacctttgcaagcacctgcacgccgagggtgcaaagttaattgtcaccgacatcaataaggaggccgtccagcgtgccgtagaggagttcggtgctacggcggtggagcctaacgaaatttatggggtggaatgcgacatttatgcgccatgtgcactcggcgcaacggtaaatgatgaaaccatcccacagcttaaagcgaaagtgattgccggcagcgctaataatcagcttaaagaagatcggcatggcgatatcattcatgaaatgggcatcgtctacgccccggattatgttattaatgcgggtggtgttattaacgtggcggatgaactgtacggctataaccgcgaacgtgcactgaaacgtgtggaaagcatttacgacaccattgccaaggtgatcgaaatctcgaaacgggatggcatcgccacgtacgtagccgcggatcggttggcggaagaacgcatcgcctcgttgaaaaacagtcgctcgacgtatctgcgcaacggccatgacatcattagtcgccggtaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1719011_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaag BBa_B0032_sequence 1 tcacacaggaaag BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K1719000_sequence 1 atgacattggaaatcttcgaatatctggaaaagtatgattatgaacaggttgtgttttgtcaggacaaagagagcggactgaaagcaattatcgccattcatgacactaccctgggaccggctctcggcggtacccgcatgtggacctatgattcagaggaagcggcaatcgaagatgcactccgtctggcaaaaggcatgacatataaaaacgcagctgctggtttaaatctgggtggcgcgaaaaccgtcattattggcgatccgcgtaaagataaatcagaagcgatgtttcgtgccctgggtcgctacatccaagggctgaacgggcgttatattaccgcggaagatgtaggcactacggttgacgatatggatatcattcacgaggagactgattttgtcacgggcatctctccgtccttcgggagctccggaaatccgtctccggttaccgcttatggggtttaccgcgggatgaaagcggcggcgaaagaggcgtttggtaccgacaatttagagggcaaagtgattgcggtgcaaggtgtgggcaatgtggcctatcacctttgcaagcacctgcacgccgagggtgcaaagttaattgtcaccgacatcaataaggaggccgtccagcgtgccgtagaggagttcggtgctacggcggtggagcctaacgaaatttatggggtggaatgcgacatttatgcgccatgtgcactcggcgcaacggtaaatgatgaaaccatcccacagcttaaagcgaaagtgattgccggcagcgctaataatcagcttaaagaagatcggcatggcgatatcattcatgaaatgggcatcgtctacgccccggattatgttattaatgcgggtggtgttattaacgtggcggatgaactgtacggctataaccgcgaacgtgcactgaaacgtgtggaaagcatttacgacaccattgccaaggtgatcgaaatctcgaaacgggatggcatcgccacgtacgtagccgcggatcggttggcggaagaacgcatcgcctcgttgaaaaacagtcgctcgacgtatctgcgcaacggccatgacatcattagtcgccggtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z