LOCUS BBa_K1719013 2841 bp DNA linear 01-Jun-2024 DEFINITION a dasd ACCESSION BBa_K1719013 VERSION BBa_K1719013.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 misc_feature 1..221 /label=BBa_K1719011 CDS 228..1328 /label=BBa_K1719000 misc_feature 1337..1375 /label=B1006 stem_loop 1346..1367 /label=modified thr terminator polyA_site 1368..1375 /label=PolyA polyA_site 1337..1345 /label=PolyA terminator 1337..1375 /label=BBa_B1006 misc_feature 228..1375 /label=BBa_K1719002 misc_feature 1..1375 /label=BBa_K1719012 CDS 1384..1471 /label=end of LacI coding region (inactive) misc_feature 1384..1583 /label=BBa_R0010 misc_feature 1472..1509 /label=CAP binding site promoter 1520..1525 /label=-35 promoter 1544..1549 /label=-10 misc_feature 1549..1583 /label=LacI binding site misc_feature 1556..1556 /label=start promoter 1384..1583 /label=BBa_R0010 misc_feature 1596..1599 /label=conserved RBS 1592..1603 /label=BBa_B0034 misc_feature 1384..1603 /label=BBa_J04500 CDS 1610..2704 /label=BBa_K1719001 stem_loop 2724..2767 /label=stem_loop misc_feature 2713..2792 /label=BBa_B0010 terminator 2713..2792 /label=BBa_B0010 stem_loop 2808..2827 /label=T7 TE misc_feature 2834..2834 /label=stop polyA_site 2828..2841 /label=polya misc_feature 2801..2841 /label=BBa_B0012 terminator 2801..2841 /label=BBa_B0012 terminator 2713..2841 /label=BBa_B0015 misc_feature 1384..2841 /label=BBa_K1719007 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg acattggaaa 241 tcttcgaata tctggaaaag tatgattatg aacaggttgt gttttgtcag gacaaagaga 301 gcggactgaa agcaattatc gccattcatg acactaccct gggaccggct ctcggcggta 361 cccgcatgtg gacctatgat tcagaggaag cggcaatcga agatgcactc cgtctggcaa 421 aaggcatgac atataaaaac gcagctgctg gtttaaatct gggtggcgcg aaaaccgtca 481 ttattggcga tccgcgtaaa gataaatcag aagcgatgtt tcgtgccctg ggtcgctaca 541 tccaagggct gaacgggcgt tatattaccg cggaagatgt aggcactacg gttgacgata 601 tggatatcat tcacgaggag actgattttg tcacgggcat ctctccgtcc ttcgggagct 661 ccggaaatcc gtctccggtt accgcttatg gggtttaccg cgggatgaaa gcggcggcga 721 aagaggcgtt tggtaccgac aatttagagg gcaaagtgat tgcggtgcaa ggtgtgggca 781 atgtggccta tcacctttgc aagcacctgc acgccgaggg tgcaaagtta attgtcaccg 841 acatcaataa ggaggccgtc cagcgtgccg tagaggagtt cggtgctacg gcggtggagc 901 ctaacgaaat ttatggggtg gaatgcgaca tttatgcgcc atgtgcactc ggcgcaacgg 961 taaatgatga aaccatccca cagcttaaag cgaaagtgat tgccggcagc gctaataatc 1021 agcttaaaga agatcggcat ggcgatatca ttcatgaaat gggcatcgtc tacgccccgg 1081 attatgttat taatgcgggt ggtgttatta acgtggcgga tgaactgtac ggctataacc 1141 gcgaacgtgc actgaaacgt gtggaaagca tttacgacac cattgccaag gtgatcgaaa 1201 tctcgaaacg ggatggcatc gccacgtacg tagccgcgga tcggttggcg gaagaacgca 1261 tcgcctcgtt gaaaaacagt cgctcgacgt atctgcgcaa cggccatgac atcattagtc 1321 gccggtaata ctagagaaaa aaaaaccccg cccctgacag ggcggggttt ttttttacta 1381 gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 1441 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 1501 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 1561 tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga tgaaaattgt 1621 cctggtcctg tatgacgcgg gcaaacatgc ggccgatgag gagaaacttt acggatctac 1681 ggaaaataaa ctggggatcg ccaattggct gaaagatcag ggccacgaac tgatcaccac 1741 aagtgataaa gaaggggaaa caagcgaatt ggataagcat attccggatg cagatatcat 1801 tattactacg ccgtttcatc cagcatatat caccaaagaa cgcctcgata aagctaagaa 1861 cctgaagttg gtggtagtcg caggggtggg gtcggatcat attgacctgg attacattaa 1921 tcagaccggg aaaaaaattt ctgtgttaga agttaccggc agtaatgtcg tttctgtggc 1981 cgaacacgtg gttatgacca tgttggttct ggtgcgcaac tttgtgccag cacatgaaca 2041 gattatcaat cacgactggg aggttgccgc gatcgcaaaa gacgcctacg atatcgaagg 2101 aaaaactatc gctactatcg gtgcgggccg catcggttat cgtgttttgg agcgtcttct 2161 gccttttaac ccgaaagagc tcttatatta cgattatcag gccttaccga aagaagcgga 2221 agagaaagta ggtgcgcgtc gtgtggaaaa tatcgaagaa ttagtagcgc aagcagatat 2281 cgtgacggtg aacgcgcctc tccatgccgg tacgaaaggc ctgattaata aggaactcct 2341 gtccaaattc aaaaaaggtg cgtggcttgt gaataccgct cgcggtgcga tttgcgtcgc 2401 tgaagacgtg gcggcagcgc tggagagcgg ccaacttcgc ggttatggcg gtgacgtatg 2461 gtttccgcag ccggctccga aagaccaccc atggcgcgac atgcgtaaca aatatggcgc 2521 gggcaacgcc atgaccccgc attattcggg taccaccctg gatgcccaaa cccggtacgc 2581 agagggcacc aagaatattc tggagtcatt tttcacgggc aaattcgatt atcggccgca 2641 ggatattatt ctgttgaacg gagagtatgt tacgaaggcc tatggcaaac acgataaaaa 2701 gtaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2761 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2821 gtgggccttt ctgcgtttat a //