##gff-version 3
##sequence-region BBa_K1719013 1 2841
BBa_K1719013	.	engineered_region	1	1375	.	+	0	ID=BBa_K1719012;Name=BBa_K1719012
BBa_K1719013	.	engineered_region	228	1375	.	+	0	ID=BBa_K1719002;Name=BBa_K1719002;Parent=BBa_K1719012
BBa_K1719013	.	CDS	228	1328	.	+	0	ID=BBa_K1719000;Name=BBa_K1719000;Parent=BBa_K1719002
BBa_K1719013	.	terminator	1337	1375	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K1719002
BBa_K1719013	.	polyA_site	1368	1375	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1719013	.	polyA_site	1337	1345	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1719013	.	engineered_region	1337	1375	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1719013	.	stem_loop	1346	1367	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1719013	.	engineered_region	1	221	.	+	0	ID=BBa_K1719011;Name=BBa_K1719011;Parent=BBa_K1719012
BBa_K1719013	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1719011
BBa_K1719013	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1719013	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1719013	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1719013	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1719013	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1719013	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1719013	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1719013	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1719011
BBa_K1719013	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1719013	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1719013	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1719013	.	engineered_region	1384	2841	.	+	0	ID=BBa_K1719007;Name=BBa_K1719007
BBa_K1719013	.	engineered_region	1384	1603	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1719007
BBa_K1719013	.	ribosome_entry_site	1592	1603	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1719013	.	conserved_region	1596	1599	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1719013	.	promoter	1384	1583	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1719013	.	non_covalent_binding_site	1549	1583	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1719013	.	non_covalent_binding_site	1472	1509	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1719013	.	engineered_region	1384	1583	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1719013	.	promoter	1520	1525	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1719013	.	CDS	1384	1471	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1719013	.	start_codon	1556	1556	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1719013	.	promoter	1544	1549	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1719013	.	CDS	1610	2704	.	+	0	ID=BBa_K1719001;Name=BBa_K1719001;Parent=BBa_K1719007
BBa_K1719013	.	terminator	2713	2841	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1719007
BBa_K1719013	.	terminator	2713	2792	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1719013	.	stem_loop	2724	2767	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1719013	.	engineered_region	2713	2792	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1719013	.	terminator	2801	2841	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1719013	.	engineered_region	2801	2841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1719013	.	stop_codon	2834	2834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1719013	.	stem_loop	2808	2827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1719013	.	polyA_site	2828	2841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K1719013
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgacattgg
aaatcttcgaatatctggaaaagtatgattatgaacaggttgtgttttgtcaggacaaagagagcggactgaaagcaat
tatcgccattcatgacactaccctgggaccggctctcggcggtacccgcatgtggacctatgattcagaggaagcggca
atcgaagatgcactccgtctggcaaaaggcatgacatataaaaacgcagctgctggtttaaatctgggtggcgcgaaaa
ccgtcattattggcgatccgcgtaaagataaatcagaagcgatgtttcgtgccctgggtcgctacatccaagggctgaa
cgggcgttatattaccgcggaagatgtaggcactacggttgacgatatggatatcattcacgaggagactgattttgtc
acgggcatctctccgtccttcgggagctccggaaatccgtctccggttaccgcttatggggtttaccgcgggatgaaag
cggcggcgaaagaggcgtttggtaccgacaatttagagggcaaagtgattgcggtgcaaggtgtgggcaatgtggccta
tcacctttgcaagcacctgcacgccgagggtgcaaagttaattgtcaccgacatcaataaggaggccgtccagcgtgcc
gtagaggagttcggtgctacggcggtggagcctaacgaaatttatggggtggaatgcgacatttatgcgccatgtgcac
tcggcgcaacggtaaatgatgaaaccatcccacagcttaaagcgaaagtgattgccggcagcgctaataatcagcttaa
agaagatcggcatggcgatatcattcatgaaatgggcatcgtctacgccccggattatgttattaatgcgggtggtgtt
attaacgtggcggatgaactgtacggctataaccgcgaacgtgcactgaaacgtgtggaaagcatttacgacaccattg
ccaaggtgatcgaaatctcgaaacgggatggcatcgccacgtacgtagccgcggatcggttggcggaagaacgcatcgc
ctcgttgaaaaacagtcgctcgacgtatctgcgcaacggccatgacatcattagtcgccggtaatactagagaaaaaaa
aaccccgcccctgacagggcggggtttttttttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattc
attaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcac
tcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcac
acatactagagaaagaggagaaatactagatgaaaattgtcctggtcctgtatgacgcgggcaaacatgcggccgatga
ggagaaactttacggatctacggaaaataaactggggatcgccaattggctgaaagatcagggccacgaactgatcacc
acaagtgataaagaaggggaaacaagcgaattggataagcatattccggatgcagatatcattattactacgccgtttc
atccagcatatatcaccaaagaacgcctcgataaagctaagaacctgaagttggtggtagtcgcaggggtggggtcgga
tcatattgacctggattacattaatcagaccgggaaaaaaatttctgtgttagaagttaccggcagtaatgtcgtttct
gtggccgaacacgtggttatgaccatgttggttctggtgcgcaactttgtgccagcacatgaacagattatcaatcacg
actgggaggttgccgcgatcgcaaaagacgcctacgatatcgaaggaaaaactatcgctactatcggtgcgggccgcat
cggttatcgtgttttggagcgtcttctgccttttaacccgaaagagctcttatattacgattatcaggccttaccgaaa
gaagcggaagagaaagtaggtgcgcgtcgtgtggaaaatatcgaagaattagtagcgcaagcagatatcgtgacggtga
acgcgcctctccatgccggtacgaaaggcctgattaataaggaactcctgtccaaattcaaaaaaggtgcgtggcttgt
gaataccgctcgcggtgcgatttgcgtcgctgaagacgtggcggcagcgctggagagcggccaacttcgcggttatggc
ggtgacgtatggtttccgcagccggctccgaaagaccacccatggcgcgacatgcgtaacaaatatggcgcgggcaacg
ccatgaccccgcattattcgggtaccaccctggatgcccaaacccggtacgcagagggcaccaagaatattctggagtc
atttttcacgggcaaattcgattatcggccgcaggatattattctgttgaacggagagtatgttacgaaggcctatggc
aaacacgataaaaagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata