LOCUS BBa_K1723000 4383 bp DNA linear 29-Nov-2024 DEFINITION dCas9-ω ACCESSION BBa_K1723000 VERSION BBa_K1723000.1 FEATURES Location/Qualifiers misc_feature 4105..4113 /label=linker misc_feature 4114..4383 /label=rpoZ (omega subunit) CDS 1..4104 /label=dCas9 ORIGIN 1 atggataaga aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg 61 atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc 121 cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa 181 gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt 241 tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga 301 cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga 361 aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa 421 aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat 481 atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat 541 gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct 601 attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga 661 cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat 721 ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa 781 gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg 841 caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt 901 ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca 961 atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga 1021 caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca 1081 ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta 1141 gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc 1201 aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat 1261 gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt 1321 gaaaaaatct tgacttttcg cattccttat tatgttggtc cattggcgcg tggcaatagt 1381 cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa 1441 gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa 1501 aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt 1561 tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt 1621 tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc 1681 gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt 1741 tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt 1801 attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt 1861 ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct 1921 cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga 1981 cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta 2041 gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat 2101 agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta 2161 catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact 2221 gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt 2281 attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt 2341 atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct 2401 gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga 2461 gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatgcc 2521 attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct 2581 gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa 2641 aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta 2701 acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa 2761 ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat 2821 actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct 2881 aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat 2941 taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa 3001 tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa 3061 atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct 3121 aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc 3181 cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt 3241 gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta 3301 cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt 3361 gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct 3421 tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt 3481 aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac 3541 tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa 3601 tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta 3661 caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt 3721 cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag 3781 cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt 3841 attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa 3901 ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct 3961 cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa 4021 gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt 4081 gatttgagtc agctaggagg tgacgcagct gcacgcgtaa ctgttcagga cgctgtagag 4141 aaaattggta accgttttga cctggtactg gtcgccgcgc gtcgcgctcg tcagatgcag 4201 gtaggcggaa aggatccgct ggtaccggaa gaaaacgata aaaccactgt aatcgcgctg 4261 cgcgaaatcg aagaaggtct gatcaacaac cagatcctcg acgttcgcga acgccaggaa 4321 cagcaagagc aggaagccgc tgaattacaa gccgttaccg ctattgctga aggtcgtcgt 4381 taa //