LOCUS       BBa_K1723003             275 bp    DNA     linear       23-Dec-2024
DEFINITION  sgRNA Z4 expressing cassette
ACCESSION   BBa_K1723003
VERSION     BBa_K1723003.1  
FEATURES             Location/Qualifiers
     promoter        1..173
                     /label=pBAD promoter
     misc_feature    174..193
                     /label=Z4 specific sequence
     misc_feature    194..235
                     /label=sgRNA scaffold
     misc_feature    236..275
                     /label=S. Pyogenes terminator
ORIGIN
        1 gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc
       61 cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga
      121 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc atagaagatc
      181 cggcctgcag ccagttttag agctagaaat agcaagttaa aataaggcta gtccgattgc
      241 aacttgaaaa gtggcaccga gtcggtgctt ttttt
//