LOCUS       BBa_K1723004             274 bp    DNA     linear       23-Dec-2024
DEFINITION  sgRNA Z35 expressing cassette
ACCESSION   BBa_K1723004
VERSION     BBa_K1723004.1  
FEATURES             Location/Qualifiers
     promoter        1..172
                     /label=pBAD promoter
     misc_feature    173..192
                     /label=Z35 specific sequence 
     misc_feature    193..234
                     /label=sgRNA scaffold
     misc_feature    235..274
                     /label=S. Pyogenes terminator
ORIGIN
        1 gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc
       61 cacattgatt atttgcacgg cgtcacactt tgtatgccat agcattttta tccataagat
      121 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taactgagct
      181 agctgtcaag acgttttaga gctagaaata gcaagttaaa ataaggctag tccgattgca
      241 acttgaaaaa gtggcaccga gcggtgcttt tttt
//