LOCUS BBa_K1723005 282 bp DNA linear 22-Nov-2024 DEFINITION PAM rich URS J23117Alt promoter ACCESSION BBa_K1723005 VERSION BBa_K1723005.1 FEATURES Location/Qualifiers misc_feature 1..247 /label=PAM rich URS misc_feature 248..282 /label=J23117Alt misc_feature 189..191 /label=PAM misc_feature 192..211 /label=sgRNA X4 recognition site misc_feature 242..244 /label=PAM misc_feature 245..264 /label=sgRNA X35 recognition site misc_feature 269..271 /label=PAM misc_feature 249..268 /label=sgRNA X0 recognition site misc_feature 271..276 /label=-10 sequence misc_feature 248..253 /label=-35 sequence ORIGIN 1 aagcagagga gcaaaagctc atttctgaag aggacttgtt gcggaaacga cgagaacagt 61 tgaaacacaa acttgaacag ctacggaact cttgtgcgta aggaaaagta aggaaaacga 121 ttccttctaa cagaaatgtc ctgagcaatc acctatgaac tgtcgactcg agcctctatg 181 gattatcacc gtccctcgag aaattgagcg ccacaacacg cacggtgtta cattaggcat 241 accttcgttg acatgctaaa gagatatcgg gattgtgcta gc //