LOCUS       BBa_K1723008             276 bp    DNA     linear       23-Dec-2024
DEFINITION  sgRNA X35 expressing cassette
ACCESSION   BBa_K1723008
VERSION     BBa_K1723008.1  
FEATURES             Location/Qualifiers
     promoter        1..173
                     /label=pBAD promoter
     misc_feature    174..193
                     /label=X35 specific sequence 
     misc_feature    194..235
                     /label=sgRNA scaffold 
     misc_feature    236..276
                     /label=S. Pyogenes terminator
ORIGIN
        1 gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc
       61 cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga
      121 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc atatctcttt
      181 agcatgtcaa cgagttttag agctagaaat agcaagttaa aataaggcta gtccgattgc
      241 aacttgaaaa agtggcaccg agtcggtgct tttttt
//