BBa_K1723009
1
BBa_K1723009
c3_0 gRNA expressing sequence
2015-09-15T11:00:00Z
2015-09-16T10:51:41Z
Synthesized
The c3_0 guide RNA (gRNA) consists of the 20 base pair specificity determinent sequence (SDS) c3_0 followed by a gRNA scaffold that stabilizes the RNA complex. The gRNA is flanked by two self-cleaving ribozymes: a Hammerhead Ribozyme and a HDV Ribozyme thus freeing the gRNA from the rest of the RNA transcript. Then, the gRNA can form a complex with dCas9-VP64 (or any other Cas9 mutant). The complex c3_0-dCas9_VP64 will bind to any GTACATACAGTAGGATCCTA sequence in the genome of the host organism situated directly before a PAM sequence (NGG).
false
false
_2143_
25334
25334
9
false
The c3_0 gRNA - dCas9_VP64 complex works as an activator of the RNA polymerase II CYC_0 promoter. The Hammerhead ribozyme - gRNA - HDV rizobyme sequence design allows to express gRNAs using RNA polymerase II, thus making it possible to chain the expression of gRNAs (by activating another RNA polymerase II promoter which can express another gRNA e.g.).
false
Cyril Pulver
annotation2461244
1
gRNA scaffold
range2461244
1
64
139
annotation2461235
1
Hammerhead Ribozyme
range2461235
1
1
43
annotation2461247
1
HDV Ribozyme
range2461247
1
144
211
annotation2461240
1
c3_0 SDS
range2461240
1
44
63
BBa_K1723009_sequence
1
cgactactgatgagtccgtgaggacgaaacgagtaagctcgtcgtacatacagtaggatcctagttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttggccggcatggtcccagcctcctcgctggcgccggctgggcaacatgcttcggcatggcgaatgggac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z