LOCUS       BBa_K1725002             795 bp    DNA     linear       22-Dec-2024
DEFINITION  PhlF repressible promoter + weak RBS + GFP
ACCESSION   BBa_K1725002
VERSION     BBa_K1725002.1  
FEATURES             Location/Qualifiers
     misc_feature    19..48
                     /label=K1725040 operator sequence
     misc_feature    14..19
                     /label=-35 site
     misc_feature    37..42
                     /label=-10 site
     promoter        1..48
                     /label=BBa_K1725000
     RBS             57..69
                     /label=RBS-3\Weak
     RBS             63..66
                     /label=RBS
     misc_feature    57..69
                     /label=BBa_B0032
     RBS             57..69
                     /label=BBa_B0032
     CDS             76..795
                     /label=GFP protein
     CDS             76..795
                     /label=BBa_E0040
     misc_feature    57..795
                     /label=BBa_E5501
ORIGIN
        1 gattcgttac caattgacat gatacgaaac gtaccgtatc gttaaggtta ctagagtcac
       61 acaggaaagt actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      121 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      181 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      241 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      301 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      361 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      421 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      481 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      541 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      601 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      661 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      721 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      781 ctatacaaat aataa
//