BBa_K1725020
1
P-srpR
SrpR repressible promoter
2015-08-06T11:00:00Z
2015-09-18T03:58:50Z
From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
The promoter is on normally, and off when the SrpR repressor (K1725061) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725000.
false
false
_2146_
20827
27284
9
false
We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html They based the promoter sequence on J23119, then overlapped the operator sequence of the SrpR repressor over the -10 site.
false
Mhairi Davidson
annotation2461859
1
K1725060 operator sequence
range2461859
1
38
67
BBa_K1725020_sequence
1
gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z