BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K1725040
1
phlF
<i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5
2015-08-10T11:00:00Z
2016-07-23T11:16:49Z
Sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
PhlF repressor from Pseudomonas protegens Pf-5. Represses promoter K1725000.
false
false
_2146_
27285
27284
9
true
.
false
Mhairi Davidson
annotation2473659
1
phlF
range2473659
1
1
600
BBa_K1725041
1
BBa_K1725041
RBS + PhlF repressor + terminator
2015-08-10T11:00:00Z
2015-08-11T10:49:59Z
.
PhlF repressor from Pseudomonas protegens Pf-5, with RBS B0032, and terminator K1725081
false
false
_2146_
27284
27284
9
false
.
false
Mhairi Davidson
component2435066
1
BBa_K1725081
component2435063
1
BBa_B0032
component2435065
1
BBa_K1725040
annotation2435066
1
BBa_K1725081
range2435066
1
631
731
annotation2435063
1
BBa_B0032
range2435063
1
1
13
annotation2435065
1
BBa_K1725040
range2435065
1
20
622
BBa_K1725081
1
BBa_K1725081
T1 terminator from E. coli rrnB
2015-08-10T11:00:00Z
2016-07-23T11:20:14Z
T1 terminator from E. coli rrnB
T1 terminator from E. coli rrnB
false
false
_2146_
27285
27284
9
true
.
false
Mhairi Davidson
annotation2476138
1
T1
range2476138
1
12
56
annotation2476137
1
B0010
range2476137
1
1
80
BBa_K1725081_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg
BBa_K1725041_sequence
1
tcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K1725040_sequence
1
atggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z