LOCUS       BBa_K1725042             800 bp    DNA     linear       11-May-2024
DEFINITION  LacI regulated promoter + RBS + PhlF repressor + terminator
ACCESSION   BBa_K1725042
VERSION     BBa_K1725042.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=NheI site
     promoter        7..61
                     /label=PLlac 0-1 promoter
     promoter        1..61
                     /label=BBa_K1725080
     RBS             70..82
                     /label=RBS-3\Weak
     RBS             76..79
                     /label=RBS
     misc_feature    70..82
                     /label=BBa_B0032
     RBS             70..82
                     /label=BBa_B0032
     CDS             89..688
                     /label=phlF
     CDS             89..691
                     /label=BBa_K1725040
     misc_feature    700..779
                     /label=B0010
     misc_feature    711..755
                     /label=T1
     terminator      700..800
                     /label=BBa_K1725081
     misc_feature    70..800
                     /label=BBa_K1725041
ORIGIN
        1 gctagcaatt gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac
       61 atactagagt cacacaggaa agtactagat ggcacgtacc ccgagccgta gcagcattgg
      121 tagcctgcgt agtccgcata cccataaagc aattctgacc agcaccattg aaatcctgaa
      181 agaatgtggt tatagcggtc tgagcattga aagcgttgca cgtcgtgccg gtgcaagcaa
      241 accgaccatt tatcgttggt ggaccaataa agcagcactg attgccgaag tgtatgaaaa
      301 tgaaagcgaa caggtgcgta aatttccgga tctgggtagc tttaaagccg atctggattt
      361 tctgctgcgt aatctgtgga aagtttggcg tgaaaccatt tgtggtgaag catttcgttg
      421 tgttattgca gaagcacagc tggaccctgc aaccctgacc cagctgaaag atcagtttat
      481 ggaacgtcgt cgtgagatgc cgaaaaaact ggttgaaaat gccattagca atggtgaact
      541 gccgaaagat accaatcgtg aactgctgct ggatatgatt tttggttttt gttggtatcg
      601 cctgctgacc gaacagctga ccgttgaaca ggatattgaa gaatttacct tcctgctgat
      661 taatggtgtt tgtccgggta cacagcgtta atactagagc caggcatcaa ataaaacgaa
      721 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
      781 tgagtaggac aaatccgccg
//