BBa_K1725040 1 phlF <i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5 2015-08-10T11:00:00Z 2016-07-23T11:16:49Z Sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html PhlF repressor from Pseudomonas protegens Pf-5. Represses promoter K1725000. false false _2146_ 27285 27284 9 true . false Mhairi Davidson annotation2473659 1 phlF range2473659 1 1 600 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_K1725041 1 BBa_K1725041 RBS + PhlF repressor + terminator 2015-08-10T11:00:00Z 2015-08-11T10:49:59Z . PhlF repressor from Pseudomonas protegens Pf-5, with RBS B0032, and terminator K1725081 false false _2146_ 27284 27284 9 false . false Mhairi Davidson component2435066 1 BBa_K1725081 component2435065 1 BBa_K1725040 component2435063 1 BBa_B0032 annotation2435065 1 BBa_K1725040 range2435065 1 20 622 annotation2435066 1 BBa_K1725081 range2435066 1 631 731 annotation2435063 1 BBa_B0032 range2435063 1 1 13 BBa_K1725080 1 R0011-N Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site 2015-08-10T11:00:00Z 2016-07-23T11:23:04Z From BBa_R0011. Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be: repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]). induced by IPTG in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range Extra Nhe1 site added, for ease of checking for BioBrick with a digest false false _2146_ 27285 27284 9 true BBa_R0011 with Nhe1 site upstream of promoter (after prefix) false Mhairi Davidson annotation2455109 1 NheI site range2455109 1 1 6 annotation2455111 1 PLlac 0-1 promoter range2455111 1 7 61 BBa_K1725042 1 BBa_K1725042 LacI regulated promoter + RBS + PhlF repressor + terminator 2015-08-10T11:00:00Z 2015-08-11T10:55:15Z . PhlF repressor from Pseudomonas protegens Pf-5, with promoter K1725080 with RBS B0032, and terminator K1725081 false false _2146_ 27284 27284 9 false . false Mhairi Davidson component2435073 1 BBa_K1725041 component2435067 1 BBa_K1725080 annotation2435073 1 BBa_K1725041 range2435073 1 70 800 annotation2435067 1 BBa_K1725080 range2435067 1 1 61 BBa_K1725081 1 BBa_K1725081 T1 terminator from E. coli rrnB 2015-08-10T11:00:00Z 2016-07-23T11:20:14Z T1 terminator from E. coli rrnB T1 terminator from E. coli rrnB false false _2146_ 27285 27284 9 true . false Mhairi Davidson annotation2476138 1 T1 range2476138 1 12 56 annotation2476137 1 B0010 range2476137 1 1 80 BBa_K1725042_sequence 1 gctagcaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg BBa_K1725081_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg BBa_K1725041_sequence 1 tcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg BBa_K1725080_sequence 1 gctagcaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_B0032_sequence 1 tcacacaggaaag BBa_K1725040_sequence 1 atggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z