BBa_K1725060
1
srpR
SrpR repressor from Pseudomonas putida
2015-08-11T11:00:00Z
2015-09-18T02:13:41Z
Sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
SrpR repressor from Pseudomonas putida. Represses promoter K1725020.
false
false
_2146_
20827
27284
9
false
Removed 3 Spe1 restriction sites to make it BioBrick compatible.
false
Mhairi Davidson
annotation2473681
1
srpR
range2473681
1
1
639
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation1709
1
RBS-3\Weak
range1709
1
1
13
BBa_K1725061
1
BBa_K1725061
<i>srpR</i> with RBS and terminator
2015-09-09T11:00:00Z
2015-09-18T02:59:07Z
.
srpR repressor (K1725060) with ribosome binding site B0032 (0.3 of B0034) and terminator K1725081
false
false
_2146_
27284
27284
9
false
.
false
Mhairi Davidson
component2448641
1
BBa_K1725060
component2448639
1
BBa_B0032
component2448642
1
BBa_K1725081
annotation2448642
1
BBa_K1725081
range2448642
1
670
770
annotation2448641
1
BBa_K1725060
range2448641
1
20
661
annotation2448639
1
BBa_B0032
range2448639
1
1
13
BBa_K1725081
1
BBa_K1725081
T1 terminator from E. coli rrnB
2015-08-10T11:00:00Z
2016-07-23T11:20:14Z
T1 terminator from E. coli rrnB
T1 terminator from E. coli rrnB
false
false
_2146_
27285
27284
9
true
.
false
Mhairi Davidson
annotation2476138
1
T1
range2476138
1
12
56
annotation2476137
1
B0010
range2476137
1
1
80
BBa_K1725060_sequence
1
atggcacgtaaaaccgcagcagaagcagaagaaacccgtcagcgtattattgatgcagcactggaagtttttgttgcacagggtgttagtgatgcaaccctggatcagattgcacgtaaagccggtgttacccgtggtgcagtttattggcattttaatggtaaactggaagttctgcaagcagttctggcaagccgtcagcatccgctggaactggattttacaccggatctgggtattgaacgtagctgggaagcagttgttgttgcaatgctggatgcagttcatagtccgcagagcaaacagtttagcgaaattctgatttatcagggtctggatgaaagcggtctgattcataatcgtatggttcaggcaagcgatcgttttctgcaatatattcatcaggttctgcgtcatgcagttacccagggtgaactgccgattaatctggatctgcaaaccagcattggtgtttttaaaggtctgattaccggtctgctgtatgaaggtctgcgtagcaaagatcagcaggcacagattatcaaagttgcactgggtagcttttgggcactgctgcgtgaaccgcctcgttttctgctgtgtgaagaagcacagattaaacaggtgaaatccttcgaataa
BBa_K1725081_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K1725061_sequence
1
tcacacaggaaagtactagatggcacgtaaaaccgcagcagaagcagaagaaacccgtcagcgtattattgatgcagcactggaagtttttgttgcacagggtgttagtgatgcaaccctggatcagattgcacgtaaagccggtgttacccgtggtgcagtttattggcattttaatggtaaactggaagttctgcaagcagttctggcaagccgtcagcatccgctggaactggattttacaccggatctgggtattgaacgtagctgggaagcagttgttgttgcaatgctggatgcagttcatagtccgcagagcaaacagtttagcgaaattctgatttatcagggtctggatgaaagcggtctgattcataatcgtatggttcaggcaagcgatcgttttctgcaatatattcatcaggttctgcgtcatgcagttacccagggtgaactgccgattaatctggatctgcaaaccagcattggtgtttttaaaggtctgattaccggtctgctgtatgaaggtctgcgtagcaaagatcagcaggcacagattatcaaagttgcactgggtagcttttgggcactgctgcgtgaaccgcctcgttttctgctgtgtgaagaagcacagattaaacaggtgaaatccttcgaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z