LOCUS       BBa_K1725062             839 bp    DNA     linear       16-Feb-2025
DEFINITION  LacI regulated promoter + RBS + SrpR repressor + terminator
ACCESSION   BBa_K1725062
VERSION     BBa_K1725062.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=NheI site
     promoter        7..61
                     /label=PLlac 0-1 promoter
     promoter        1..61
                     /label=BBa_K1725080
     RBS             70..82
                     /label=RBS-3\Weak
     RBS             76..79
                     /label=RBS
     misc_feature    70..82
                     /label=BBa_B0032
     RBS             70..82
                     /label=BBa_B0032
     CDS             89..727
                     /label=srpR
     CDS             89..730
                     /label=BBa_K1725060
     misc_feature    739..818
                     /label=B0010
     misc_feature    750..794
                     /label=T1
     terminator      739..839
                     /label=BBa_K1725081
     misc_feature    70..839
                     /label=BBa_K1725061
ORIGIN
        1 gctagcaatt gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac
       61 atactagagt cacacaggaa agtactagat ggcacgtaaa accgcagcag aagcagaaga
      121 aacccgtcag cgtattattg atgcagcact ggaagttttt gttgcacagg gtgttagtga
      181 tgcaaccctg gatcagattg cacgtaaagc cggtgttacc cgtggtgcag tttattggca
      241 ttttaatggt aaactggaag ttctgcaagc agttctggca agccgtcagc atccgctgga
      301 actggatttt acaccggatc tgggtattga acgtagctgg gaagcagttg ttgttgcaat
      361 gctggatgca gttcatagtc cgcagagcaa acagtttagc gaaattctga tttatcaggg
      421 tctggatgaa agcggtctga ttcataatcg tatggttcag gcaagcgatc gttttctgca
      481 atatattcat caggttctgc gtcatgcagt tacccagggt gaactgccga ttaatctgga
      541 tctgcaaacc agcattggtg tttttaaagg tctgattacc ggtctgctgt atgaaggtct
      601 gcgtagcaaa gatcagcagg cacagattat caaagttgca ctgggtagct tttgggcact
      661 gctgcgtgaa ccgcctcgtt ttctgctgtg tgaagaagca cagattaaac aggtgaaatc
      721 cttcgaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      781 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccg
//