LOCUS       BBa_K1725063             826 bp    DNA     linear       22-Dec-2024
DEFINITION  PhlF repressible promoter + weak RBS + SrpR + terminator
ACCESSION   BBa_K1725063
VERSION     BBa_K1725063.1  
FEATURES             Location/Qualifiers
     misc_feature    19..48
                     /label=K1725040 operator sequence
     misc_feature    14..19
                     /label=-35 site
     misc_feature    37..42
                     /label=-10 site
     promoter        1..48
                     /label=BBa_K1725000
     RBS             57..69
                     /label=RBS-3\Weak
     RBS             63..66
                     /label=RBS
     misc_feature    57..69
                     /label=BBa_B0032
     RBS             57..69
                     /label=BBa_B0032
     CDS             76..714
                     /label=srpR
     CDS             76..717
                     /label=BBa_K1725060
     misc_feature    726..805
                     /label=B0010
     misc_feature    737..781
                     /label=T1
     terminator      726..826
                     /label=BBa_K1725081
     misc_feature    57..826
                     /label=BBa_K1725061
ORIGIN
        1 gattcgttac caattgacat gatacgaaac gtaccgtatc gttaaggtta ctagagtcac
       61 acaggaaagt actagatggc acgtaaaacc gcagcagaag cagaagaaac ccgtcagcgt
      121 attattgatg cagcactgga agtttttgtt gcacagggtg ttagtgatgc aaccctggat
      181 cagattgcac gtaaagccgg tgttacccgt ggtgcagttt attggcattt taatggtaaa
      241 ctggaagttc tgcaagcagt tctggcaagc cgtcagcatc cgctggaact ggattttaca
      301 ccggatctgg gtattgaacg tagctgggaa gcagttgttg ttgcaatgct ggatgcagtt
      361 catagtccgc agagcaaaca gtttagcgaa attctgattt atcagggtct ggatgaaagc
      421 ggtctgattc ataatcgtat ggttcaggca agcgatcgtt ttctgcaata tattcatcag
      481 gttctgcgtc atgcagttac ccagggtgaa ctgccgatta atctggatct gcaaaccagc
      541 attggtgttt ttaaaggtct gattaccggt ctgctgtatg aaggtctgcg tagcaaagat
      601 cagcaggcac agattatcaa agttgcactg ggtagctttt gggcactgct gcgtgaaccg
      661 cctcgttttc tgctgtgtga agaagcacag attaaacagg tgaaatcctt cgaataatac
      721 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      781 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccg
//