BBa_K1725080 1 R0011-N Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site 2015-08-10T11:00:00Z 2016-07-23T11:23:04Z From BBa_R0011. Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be: repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]). induced by IPTG in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range Extra Nhe1 site added, for ease of checking for BioBrick with a digest false false _2146_ 27285 27284 9 true BBa_R0011 with Nhe1 site upstream of promoter (after prefix) false Mhairi Davidson annotation2455111 1 PLlac 0-1 promoter range2455111 1 7 61 annotation2455109 1 NheI site range2455109 1 1 6 BBa_K1725080_sequence 1 gctagcaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z