LOCUS       BBa_K1725100            1640 bp    DNA     linear       27-Dec-2024
DEFINITION  SrpR repressible promoter + weak RBS + PhlF repressor + terminator 
            + PhlF repressible promoter + wea
ACCESSION   BBa_K1725100
VERSION     BBa_K1725100.1  
FEATURES             Location/Qualifiers
     misc_feature    38..67
                     /label=K1725060 operator sequence
     promoter        1..67
                     /label=BBa_K1725020
     RBS             76..88
                     /label=RBS-3\Weak
     RBS             82..85
                     /label=RBS
     misc_feature    76..88
                     /label=BBa_B0032
     RBS             76..88
                     /label=BBa_B0032
     CDS             95..694
                     /label=phlF
     CDS             95..697
                     /label=BBa_K1725040
     misc_feature    706..785
                     /label=B0010
     misc_feature    717..761
                     /label=T1
     terminator      706..806
                     /label=BBa_K1725081
     misc_feature    76..806
                     /label=BBa_K1725041
     misc_feature    1..806
                     /label=BBa_K1725043
     misc_feature    833..862
                     /label=K1725040 operator sequence
     misc_feature    828..833
                     /label=-35 site
     misc_feature    851..856
                     /label=-10 site
     promoter        815..862
                     /label=BBa_K1725000
     RBS             871..883
                     /label=RBS-3\Weak
     RBS             877..880
                     /label=RBS
     misc_feature    871..883
                     /label=BBa_B0032
     RBS             871..883
                     /label=BBa_B0032
     CDS             890..1528
                     /label=srpR
     CDS             890..1531
                     /label=BBa_K1725060
     misc_feature    1540..1619
                     /label=B0010
     misc_feature    1551..1595
                     /label=T1
     terminator      1540..1640
                     /label=BBa_K1725081
     misc_feature    871..1640
                     /label=BBa_K1725061
     misc_feature    815..1640
                     /label=BBa_K1725063
ORIGIN
        1 gattcgttac caattgacag ctagctcagt cctaggtata tacatacatg cttgtttgtt
       61 tgtaaactac tagagtcaca caggaaagta ctagatggca cgtaccccga gccgtagcag
      121 cattggtagc ctgcgtagtc cgcataccca taaagcaatt ctgaccagca ccattgaaat
      181 cctgaaagaa tgtggttata gcggtctgag cattgaaagc gttgcacgtc gtgccggtgc
      241 aagcaaaccg accatttatc gttggtggac caataaagca gcactgattg ccgaagtgta
      301 tgaaaatgaa agcgaacagg tgcgtaaatt tccggatctg ggtagcttta aagccgatct
      361 ggattttctg ctgcgtaatc tgtggaaagt ttggcgtgaa accatttgtg gtgaagcatt
      421 tcgttgtgtt attgcagaag cacagctgga ccctgcaacc ctgacccagc tgaaagatca
      481 gtttatggaa cgtcgtcgtg agatgccgaa aaaactggtt gaaaatgcca ttagcaatgg
      541 tgaactgccg aaagatacca atcgtgaact gctgctggat atgatttttg gtttttgttg
      601 gtatcgcctg ctgaccgaac agctgaccgt tgaacaggat attgaagaat ttaccttcct
      661 gctgattaat ggtgtttgtc cgggtacaca gcgttaatac tagagccagg catcaaataa
      721 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      781 ctctcctgag taggacaaat ccgccgtact agaggattcg ttaccaattg acatgatacg
      841 aaacgtaccg tatcgttaag gttactagag tcacacagga aagtactaga tggcacgtaa
      901 aaccgcagca gaagcagaag aaacccgtca gcgtattatt gatgcagcac tggaagtttt
      961 tgttgcacag ggtgttagtg atgcaaccct ggatcagatt gcacgtaaag ccggtgttac
     1021 ccgtggtgca gtttattggc attttaatgg taaactggaa gttctgcaag cagttctggc
     1081 aagccgtcag catccgctgg aactggattt tacaccggat ctgggtattg aacgtagctg
     1141 ggaagcagtt gttgttgcaa tgctggatgc agttcatagt ccgcagagca aacagtttag
     1201 cgaaattctg atttatcagg gtctggatga aagcggtctg attcataatc gtatggttca
     1261 ggcaagcgat cgttttctgc aatatattca tcaggttctg cgtcatgcag ttacccaggg
     1321 tgaactgccg attaatctgg atctgcaaac cagcattggt gtttttaaag gtctgattac
     1381 cggtctgctg tatgaaggtc tgcgtagcaa agatcagcag gcacagatta tcaaagttgc
     1441 actgggtagc ttttgggcac tgctgcgtga accgcctcgt tttctgctgt gtgaagaagc
     1501 acagattaaa caggtgaaat ccttcgaata atactagagc caggcatcaa ataaaacgaa
     1561 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     1621 tgagtaggac aaatccgccg
//