BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_K1725020 1 P-srpR SrpR repressible promoter 2015-08-06T11:00:00Z 2015-09-18T03:58:50Z From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html The promoter is on normally, and off when the SrpR repressor (K1725061) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725000. false false _2146_ 20827 27284 9 false We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html They based the promoter sequence on J23119, then overlapped the operator sequence of the SrpR repressor over the -10 site. false Mhairi Davidson annotation2461859 1 K1725060 operator sequence range2461859 1 38 67 BBa_K1725041 1 BBa_K1725041 RBS + PhlF repressor + terminator 2015-08-10T11:00:00Z 2015-08-11T10:49:59Z . PhlF repressor from Pseudomonas protegens Pf-5, with RBS B0032, and terminator K1725081 false false _2146_ 27284 27284 9 false . false Mhairi Davidson component2435065 1 BBa_K1725040 component2435063 1 BBa_B0032 component2435066 1 BBa_K1725081 annotation2435063 1 BBa_B0032 range2435063 1 1 13 annotation2435066 1 BBa_K1725081 range2435066 1 631 731 annotation2435065 1 BBa_K1725040 range2435065 1 20 622 BBa_K1725000 1 P-phlF PhlF repressible promoter 2015-08-05T11:00:00Z 2016-07-23T11:16:25Z From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020. false false _2146_ 27285 27284 9 true We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html They based the promoter sequence on J23119, then overlapped the operator sequence of the PhlF repressor over the -10 site. false Mhairi Davidson annotation2461855 1 K1725040 operator sequence range2461855 1 19 48 annotation2476166 1 -35 site range2476166 1 14 19 annotation2476167 1 -10 site range2476167 1 37 42 BBa_K1725061 1 BBa_K1725061 <i>srpR</i> with RBS and terminator 2015-09-09T11:00:00Z 2015-09-18T02:59:07Z . srpR repressor (K1725060) with ribosome binding site B0032 (0.3 of B0034) and terminator K1725081 false false _2146_ 27284 27284 9 false . false Mhairi Davidson component2448639 1 BBa_B0032 component2448642 1 BBa_K1725081 component2448641 1 BBa_K1725060 annotation2448642 1 BBa_K1725081 range2448642 1 670 770 annotation2448639 1 BBa_B0032 range2448639 1 1 13 annotation2448641 1 BBa_K1725060 range2448641 1 20 661 BBa_K1725043 1 BBa_K1725043 SrpR repressible promoter + weak RBS + PhlF + terminator 2015-09-09T11:00:00Z 2015-09-18T03:56:02Z . . false false _2146_ 27310 27284 9 false . false Mhairi Davidson component2448631 1 BBa_K1725041 component2448625 1 BBa_K1725020 annotation2448625 1 BBa_K1725020 range2448625 1 1 67 annotation2448631 1 BBa_K1725041 range2448631 1 76 806 BBa_K1725100 1 BBa_K1725100 SrpR repressible promoter + weak RBS + PhlF repressor + terminator + PhlF repressible promoter + wea 2015-09-09T11:00:00Z 2015-09-18T04:05:17Z . . false false _2146_ 27310 27284 9 false . false Mhairi Davidson component2448693 1 BBa_K1725063 component2448685 1 BBa_K1725043 annotation2448685 1 BBa_K1725043 range2448685 1 1 806 annotation2448693 1 BBa_K1725063 range2448693 1 815 1640 BBa_K1725081 1 BBa_K1725081 T1 terminator from E. coli rrnB 2015-08-10T11:00:00Z 2016-07-23T11:20:14Z T1 terminator from E. coli rrnB T1 terminator from E. coli rrnB false false _2146_ 27285 27284 9 true . false Mhairi Davidson annotation2476138 1 T1 range2476138 1 12 56 annotation2476137 1 B0010 range2476137 1 1 80 BBa_K1725063 1 BBa_K1725063 PhlF repressible promoter + weak RBS + SrpR + terminator 2015-09-09T11:00:00Z 2015-09-18T03:54:38Z . . false false _2146_ 27310 27284 9 false . false Mhairi Davidson component2448656 1 BBa_K1725061 component2448650 1 BBa_K1725000 annotation2448650 1 BBa_K1725000 range2448650 1 1 48 annotation2448656 1 BBa_K1725061 range2448656 1 57 826 BBa_K1725040 1 phlF <i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5 2015-08-10T11:00:00Z 2016-07-23T11:16:49Z Sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html PhlF repressor from Pseudomonas protegens Pf-5. Represses promoter K1725000. false false _2146_ 27285 27284 9 true . false Mhairi Davidson annotation2473659 1 phlF range2473659 1 1 600 BBa_K1725060 1 srpR SrpR repressor from Pseudomonas putida 2015-08-11T11:00:00Z 2015-09-18T02:13:41Z Sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html SrpR repressor from Pseudomonas putida. Represses promoter K1725020. false false _2146_ 20827 27284 9 false Removed 3 Spe1 restriction sites to make it BioBrick compatible. false Mhairi Davidson annotation2473681 1 srpR range2473681 1 1 639 BBa_K1725043_sequence 1 gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaactactagagtcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg BBa_K1725060_sequence 1 atggcacgtaaaaccgcagcagaagcagaagaaacccgtcagcgtattattgatgcagcactggaagtttttgttgcacagggtgttagtgatgcaaccctggatcagattgcacgtaaagccggtgttacccgtggtgcagtttattggcattttaatggtaaactggaagttctgcaagcagttctggcaagccgtcagcatccgctggaactggattttacaccggatctgggtattgaacgtagctgggaagcagttgttgttgcaatgctggatgcagttcatagtccgcagagcaaacagtttagcgaaattctgatttatcagggtctggatgaaagcggtctgattcataatcgtatggttcaggcaagcgatcgttttctgcaatatattcatcaggttctgcgtcatgcagttacccagggtgaactgccgattaatctggatctgcaaaccagcattggtgtttttaaaggtctgattaccggtctgctgtatgaaggtctgcgtagcaaagatcagcaggcacagattatcaaagttgcactgggtagcttttgggcactgctgcgtgaaccgcctcgttttctgctgtgtgaagaagcacagattaaacaggtgaaatccttcgaataa BBa_K1725020_sequence 1 gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaac BBa_K1725100_sequence 1 gattcgttaccaattgacagctagctcagtcctaggtatatacatacatgcttgtttgtttgtaaactactagagtcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgtactagaggattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggttactagagtcacacaggaaagtactagatggcacgtaaaaccgcagcagaagcagaagaaacccgtcagcgtattattgatgcagcactggaagtttttgttgcacagggtgttagtgatgcaaccctggatcagattgcacgtaaagccggtgttacccgtggtgcagtttattggcattttaatggtaaactggaagttctgcaagcagttctggcaagccgtcagcatccgctggaactggattttacaccggatctgggtattgaacgtagctgggaagcagttgttgttgcaatgctggatgcagttcatagtccgcagagcaaacagtttagcgaaattctgatttatcagggtctggatgaaagcggtctgattcataatcgtatggttcaggcaagcgatcgttttctgcaatatattcatcaggttctgcgtcatgcagttacccagggtgaactgccgattaatctggatctgcaaaccagcattggtgtttttaaaggtctgattaccggtctgctgtatgaaggtctgcgtagcaaagatcagcaggcacagattatcaaagttgcactgggtagcttttgggcactgctgcgtgaaccgcctcgttttctgctgtgtgaagaagcacagattaaacaggtgaaatccttcgaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg BBa_K1725081_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg BBa_K1725041_sequence 1 tcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg BBa_K1725000_sequence 1 gattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggt BBa_B0032_sequence 1 tcacacaggaaag BBa_K1725061_sequence 1 tcacacaggaaagtactagatggcacgtaaaaccgcagcagaagcagaagaaacccgtcagcgtattattgatgcagcactggaagtttttgttgcacagggtgttagtgatgcaaccctggatcagattgcacgtaaagccggtgttacccgtggtgcagtttattggcattttaatggtaaactggaagttctgcaagcagttctggcaagccgtcagcatccgctggaactggattttacaccggatctgggtattgaacgtagctgggaagcagttgttgttgcaatgctggatgcagttcatagtccgcagagcaaacagtttagcgaaattctgatttatcagggtctggatgaaagcggtctgattcataatcgtatggttcaggcaagcgatcgttttctgcaatatattcatcaggttctgcgtcatgcagttacccagggtgaactgccgattaatctggatctgcaaaccagcattggtgtttttaaaggtctgattaccggtctgctgtatgaaggtctgcgtagcaaagatcagcaggcacagattatcaaagttgcactgggtagcttttgggcactgctgcgtgaaccgcctcgttttctgctgtgtgaagaagcacagattaaacaggtgaaatccttcgaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg BBa_K1725040_sequence 1 atggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaa BBa_K1725063_sequence 1 gattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggttactagagtcacacaggaaagtactagatggcacgtaaaaccgcagcagaagcagaagaaacccgtcagcgtattattgatgcagcactggaagtttttgttgcacagggtgttagtgatgcaaccctggatcagattgcacgtaaagccggtgttacccgtggtgcagtttattggcattttaatggtaaactggaagttctgcaagcagttctggcaagccgtcagcatccgctggaactggattttacaccggatctgggtattgaacgtagctgggaagcagttgttgttgcaatgctggatgcagttcatagtccgcagagcaaacagtttagcgaaattctgatttatcagggtctggatgaaagcggtctgattcataatcgtatggttcaggcaagcgatcgttttctgcaatatattcatcaggttctgcgtcatgcagttacccagggtgaactgccgattaatctggatctgcaaaccagcattggtgtttttaaaggtctgattaccggtctgctgtatgaaggtctgcgtagcaaagatcagcaggcacagattatcaaagttgcactgggtagcttttgggcactgctgcgtgaaccgcctcgttttctgctgtgtgaagaagcacagattaaacaggtgaaatccttcgaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z